Variation between different replicates in movement assays of mock- and WT-infected larvae at one, two and three dpi.

<p>Minimum, maximum and mean (± SE) distances moved of all replicates are displayed.</p

The <i>ptp</i> gene is present in all Alphabaculovirus group I NPVs.

<p>Bayesian phylogeny of baculoviruses based on the <i>lef-8</i> gene. GenBank accession numbers are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046933#pone.0046933.s003" target="_blank">Table S3</a>. Numbers in bold indicate maximum likelihood bootstrap values based on 100 replicates, while plain numbers depict Bayesian posterior probabilities. Only values ≥50 are indicated for both analyses. The bar at the bottom indicates a branch length of 10% distance. Baculoviruses possessing a <i>ptp</i> gene are marked by a black dot, while baculoviruses possessing a <i>ptp2</i> gene are marked by a black diamond.</p

The baculovirus <i>ptp</i> gene was presumably acquired from a lepidopteran host by an ancestral NPV.

<p>Bayesian phylogeny of <i>ptp</i> nucleotide sequences from diverse invertebrate taxa. GenBank and Butterflybase/InsectaCentral accession numbers are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046933#pone.0046933.s002" target="_blank">Table S2</a>. Numbers in bold indicate maximum likelihood bootstrap values based on 100 replicates, while plain numbers depict Bayesian posterior probabilities. Only values ≥50 are indicated for both analyses. The bar at the bottom indicates a branch length of 10% distance.</p

The AcMNPV <i>ptp</i> gene is expressed in the WT-, repair- and catmut-infected larvae.

<p>RT-PCR analysis on mock- (M), WT- (W), Δ<i>ptp</i>- (Δ), repair- (R) and catmut-infected (C) larvae. Expression of the AcMNPV <i>ptp</i> gene, the AcMNPV <i>ie1</i> gene and the host <i>Se</i>-<i>eIF5A</i> gene was analyzed. For each RT sample, a PCR without RT step (non-RT) was performed in parallel. For each primer pair, a no-template control was processed (-). The GeneRuler 100 bp ladder (Fermentas) was included in the agarose gel to estimate PCR fragment sizes.</p

Overview of the recombinant bacmids used in this study.

<p>On the left the names of the recombinant bacmids are indicated, while on the right the corresponding abbreviated names as they are used in this paper are shown.</p

Variation between different replicates in movement assays of mock-, WT-, Δ<i>ptp</i>-, repair- and catmut-infected larvae at three dpi.

<p>Minimum, maximum and mean (± SE) distances moved of all replicates are displayed.</p