Nuclear Envelope Associated Tether (NEAT) system

Creating transgenic plants relies on the random insertion of genes into DNA. Insert position within the genome can play a huge role in the expression level of the transgene. In other model systems, studies have shown that the physical position of the chromatin within the nucleus may alter gene expression. Chromatin positioning at the nuclear pore (NPC) is associated with enhanced expression of genes. We have used a LacO-LacI system to tether a luciferase reporter gene to the NPC and are checking expression levels by luciferase assays

Rurality and Tourism in Transition: How Digitalization Transforms the Character and Landscape of the Tourist Economy in Rural Morocco

The character of rural Morocco is changing due to increasing tourism and social media usage. This paper outlines the different consequences of ICT usage among people working in the tourism sector as part of the transitional economy in a remote area. In this region, tourism has grown into one major income sources for a few valley inhabitants – mostly men with a school education, digital and language skills, and who are financially stable. As this transitional economy evolves alongside digitalization and ICT usage and therefore a change of the region’s rural character, it leads to challenges and concerns for the local population. This ethnographic study analyzes the interdependence of increasing tourism through digitalization and the notion of rurality as a resource from a sociotechnical perspective

Using visual methods to understand physical activity maintenance following cardiac rehabilitation

© 2015 Hardcastle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Few studies have explored the factors associated with long-term maintenance of exercise following cardiac rehabilitation. The present study used auto-photography and interviews to explore the factors that influence motivation and continued participation in physical activity among post cardiac rehabilitation patients. Twenty-three semi-structured interviews were conducted alongside participant-selected photographs or drawings with participants that had continued participation in physical activity for at least two years following the cardiac rehabilitation programme. Participants were recruited from circuit training classes in East Sussex in the UK. Thematic content analysis revealed seven main themes: fear of death and ill health avoidance, critical incidents, overcoming aging, social influences, being able to enjoy life, provision of routine and structure, enjoyment and psychological well-being. Fear of death, illness avoidance, overcoming aging, and being able to enjoy life were powerful motives for continued participation in exercise. The social nature of the exercise class was also identified as a key facilitator of continued participation. Group-based exercise suited those that continued exercise participation post cardiac rehabilitation and fostered adherence

Kingmaking : the historiography of Bruce and Lancaster in royal biography, ceremonial and document

This thesis was digitised by the British Library from microfilm. You can acquire a single copy of this thesis for research purposes by clicking on the padlock icon on the thesis file. Please be aware that the text in the supplied thesis pdf file may not be as clear as text in a thesis that was born digital or digitised directly from paper due to the conversion in format. However, all of the theses in Apollo that were digitised from microfilm are readable and have been processed by optical character recognition (OCR) technology which means the reader can search and find text within the document. If you are the author of this thesis and would like to make your work openly available, please contact us: [email protected]

'Technology is Everywhere, we have the Opportunity to Learn it in the Valley': The Appropriation of a Socio-Technical Enabling Infrastructure in the Moroccan High Atlas

This paper describes the appropriation processes involved in establishing a socio-technical enabling infrastructure in a valley in the High Atlas of Morocco. We focus on the challenges of co-establishing such an intervention in a rural/mountainous region that is already undergoing a process of continuous development and profound transformation. We reflect upon the changes and unforeseen appropriation by our local partners and inhabitants in the valley of a computer club primarily used as an informal learning centre for school children. We followed an ethnographic approach and combined research perspectives from both socio-informatics and anthropology. This paper sheds light on what a successful cooperation and intervention in this kind of challenging environment can look like. It does this by taking seriously competing expectations, fragile infrastructural foundations and the socio-cultural context. Despite the challenges, the intervention managed to lead to the establishment of a socio-technical enabling infrastructure that plays a particularly valuable role in local educational endeavours and that is now moving towards supporting other members of the community. The paper thus provides insights regarding what has to be considered to create a mutually beneficial cooperation with all relevant stakeholders as well as a sustainable intervention

Bohring-Opitz (Oberklaid-Danks) syndrome: clinical study, review of the literature, and discussion of possible pathogenesis

Bohring-Opitz syndrome (BOS) is a rare congenital disorder of unknown etiology diagnosed on the basis of distinctive clinical features. We suggest diagnostic criteria for this condition, describe ten previously unreported patients, and update the natural history of four previously reported patients. This is the largest series reported to date, providing a unique opportunity to document the key clinical features and course through childhood. Investigations undertaken to try and elucidate the underlying pathogenesis of BOS using array comparative genomic hybridization and tandem mass spectrometry of cholesterol precursors did not show any pathogenic changes responsibl