The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla

Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film

Quantifying the mass transfer from mountain ranges to deposition in sedimentary basins: Source to sink studies in the Danube Basin – Black Sea system

A source to sink system describes the natural link between mountains, plains and deltas, by analysing the (re)distribution of material at shallow crustal depth and at the Earth's surface, exploring the links between coupled tectonic and surface processes. Sediment fluxes are the product of erosion and movement of material in and from sources (mountains), the transport and movement of sediments and solutes by river systems to the plains, and deposition and storage in sink zones. The ESF-EUROCORES TOPO-EUROPE SourceSink programme is a fully integrated research effort to significantly advance our predictive capabilities on the quantitative analyses of coupled active and past drainage systems by means of step-wise 4D reconstructions of sediments mass transfer, integrating geophysics, geology, geomorphology, state of the art high-resolution dating, and numerical and analogue modelling. The area selected for this programme is the Danube River Basin–Black Sea source to sink system, a world-class natural laboratory that is uniquely suited in the heart of Europe's topography, covering almost half of its surface, providing opportunities for excellent field sites to study in integration surface and subsurface data that cover the complete chain of source, carrier and sink. Quantifying and modelling the complete system in relation to the controlling parameters has resulted in significant understanding of forcing factors and linking temporal and spatial scales across multiple orogen and basin systems. This research has provided the opportunity to widen the geographical scope to other natural scenarios, where a number of mountain chains with similar geodynamic genesis separate sedimentary basins with comparable evolution