33 research outputs found
Signal exchange between endophytes and hosts : metabolic consequences that can influence food quality and postharvest storage
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Endophytic Pseudomonas fluorescens provides resistance to fungal pathogens in strawberry
vokMyynti MTT Tietopalvelu
Mansikan sienitautien kestävyyden vahvistaminen endofyyttisellä bakteerilla
vokMyynti MTT Tietopalvelut 31600 Jokioine
FinMed 2006 : proceedings of the 2nd international conference on bioreactor technology in cell, tissue culture and biomedical applications : with an additional theme molecular farming
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EBA 2005 : proceedings of the 1st international conference on plant microbe interactions : endophytes and biocontrol agents
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The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the crassulacean acid metabolism plant Kalanchöe daigremontiana
In C3 plants, the metabolite fructose 2,6-bisphosphate (Fru 2,6-P2) has an important role in the regulation of carbon partitioning during photosynthesis. To investigate the impact of Fru 2,6-P2 on carbon metabolism during Crassulacean acid metabolism (CAM), we have developed an Agrobacterium tumefaciens-mediated transformation system in order to alter genetically the obligate CAM plant Kalanchöe daigremontiana. To our knowledge, this is the first report to use genetic manipulation of a CAM species to increase our understanding of this important form of plant metabolism. Transgenic plants were generated containing a modified rat liver 6-phosphofructo-2-kinase gene. In the plants analyzed the activity of 6-phosphofructo-2-kinase ranged from 175% to 198% of that observed in wild-type plants, resulting in Fru 2,6-P2 concentrations that were 228% to 350% of wild-type plants after 2 h of illumination. A range of metabolic measurements were made on these transgenic plants to investigate the possible roles of Fru 2,6-P2 during Suc, starch, and malic acid metabolism across the deacidification period of CAM. The results suggest that Fru 2,6-P2 plays a major role in regulating partitioning between Suc and starch synthesis during photosynthesis. However, alterations in Fru 2,6-P2 levels had little effect on malate mobilization during CAM fluxes
Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor
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