74 research outputs found
In Pursuit of Affordable Health Care: On the Ground Lessons From Families in Massachusetts
Based on focus group discussions, compares the coverage and healthcare costs of families with employer-sponsored insurance and those without after the state's 2006 healthcare reform. Draws lessons on eligibility rules, enrollment procedures, and outreach
Approaches to Covering the Uninsured: A Guide
Explains the main options for expanding heath insurance coverage -- strengthening the current system, making it more affordable or more accessible, and changing how it is financed -- and their potential impact. Compares current reform proposals
Health Coverage and Access to Care for Hispanics in "New Growth Communities" and "Major Hispanic Centers"
Examines healthcare coverage for Hispanics when they migrate from cities to smaller urban and rural areas, and encounter roadblocks such as language barriers, lack of insurance, and access to health clinics
The Role of the Basic Health Program in the Coverage Continuum: Opportunities, Risks and Considerations for States
Outlines issues for offering subsidized coverage to those eligible for insurance exchange subsidies by using federal dollars that would otherwise go to those subsidies, including continuity of coverage, impact on exchanges, and financial feasibility
Medicaid and Community Health Centers: The Relationship between Coverage for Adults and Primary Care Capacity in Medically Underserved Communities
Community health centers play an important role in providing care to uninsured and low-income individuals living in medically underserved communities. They rely on many different revenue sources and, over time, Medicaid has become a central source of funding for most health centers. To better understand how Medicaid influences health center practice, this paper compares the strength of health centers in states that have expanded Medicaid coverage for adults to health centers in states with more limited Medicaid coverage for adults. Differences between the two groups of health centers help shed light on the implications of broader Medicaid coverage for low-income populations on the overall strength of health care safety net
Conjugation site heterogeneity causes variable electrostatic properties in Fc conjugates
Immunoconjugates, including antibody-drug conjugates and Fc-conjugates, represent a rapidly growing class of therapeutics undergoing clinical development. Despite their growing popularity, the high intrinsic heterogeneity of immunoconjugates often complicates the development process and limits their widespread application. In particular, immunoconjugate charge variants exhibit markedly different colloidal stabilities, solubilities, pharmacokinetics and tissue distributions. Charge variants arise spontaneously due to degradation and, depending on the type of drug, linker and conjugation site, through drug conjugation. Electrostatic changes in naked antibodies often result in poor performance characteristics, and therefore charge alterations due to degradation are critical to control. Charge properties are expected to be equally important to producing well-behaved ADCs. Charge-based methods of analysis, such as isoelectric focusing and ion exchange chromatography, are capable of probing the underlying complexities within immunoconjugate drug products. Despite the utility of these methods, there are only a few published reports of charge-based assays applied to immunoconjugates. In the present study, we sought to identify the effects of chemical conjugation on the electrostatic properties of Fc-conjugates. In order to minimize the effects of post-translational modifications (e.g. deamidation), a single Fc charge variant was isolated prior to conjugation of a fluorescent probe, Alexa Fluor 350, to the side chains of lysine residues. The resulting Fc-conjugates were assessed by a variety of analytical techniques, including isoelectric focusing and ion exchange chromatography, to determine their charge properties
The Vehicle, Spring 1999
Vol. 40, No. 2
Table of Contents
Poetry
Eve\u27s DaughterSylvia Whippopage 1
When We Wore Canoes On Our ShouldersMandy Watsonpage 2
This Is Not A Poem About GrandpaJake Tolbertpage 3
Old relationshipsBrandi Kinneypage 5
UntitledErin Winnerpage 6
BraverySylvia Whippopage 6
deep dark closetNicole Smithpage 7
Belly EarthTara Coburnpage 9
The River and FireJake Tolbertpage 10
UntitledAutumn Williamspage 12
Action PotentialKim Evanspage 13
Chimerical (a song for children)D.M. Attrapepage 14
UntitledAutumn Williamspage 16
UntitledMatthew Armstrongpage 18
Building YouSylvia Whippopage 19
RunningKim Evanspage 20
Walking Jenn to WorkJake Tolbertpage 22
Looking InKim Hunterpage 23
Void Between Me and WisconsinMandy Watsonpage 24
Artwork
UntitledWendy Finchpage 4
MeditationJennifer Lundpage 8
UntitledSteve Drakepage 15
MemoriesJennifer Lundpage 21
UntitledKathryn Kolasinskipage 25
Prose
FoundKim Hunterpage 26
A Day in the Life of William Baxter, DriverDaniel Fitzgeraldpage 32https://thekeep.eiu.edu/vehicle/1072/thumbnail.jp
The Vehicle, Spring 1999
Vol. 40, No. 2
Table of Contents
Poetry
Eve\u27s DaughterSylvia Whippopage 1
When We Wore Canoes On Our ShouldersMandy Watsonpage 2
This Is Not A Poem About GrandpaJake Tolbertpage 3
Old relationshipsBrandi Kinneypage 5
UntitledErin Winnerpage 6
BraverySylvia Whippopage 6
deep dark closetNicole Smithpage 7
Belly EarthTara Coburnpage 9
The River and FireJake Tolbertpage 10
UntitledAutumn Williamspage 12
Action PotentialKim Evanspage 13
Chimerical (a song for children)D.M. Attrapepage 14
UntitledAutumn Williamspage 16
UntitledMatthew Armstrongpage 18
Building YouSylvia Whippopage 19
RunningKim Evanspage 20
Walking Jenn to WorkJake Tolbertpage 22
Looking InKim Hunterpage 23
Void Between Me and WisconsinMandy Watsonpage 24
Artwork
UntitledWendy Finchpage 4
MeditationJennifer Lundpage 8
UntitledSteve Drakepage 15
MemoriesJennifer Lundpage 21
UntitledKathryn Kolasinskipage 25
Prose
FoundKim Hunterpage 26
A Day in the Life of William Baxter, DriverDaniel Fitzgeraldpage 32https://thekeep.eiu.edu/vehicle/1072/thumbnail.jp
Vinculin phosphorylation differentially regulates mechanotransduction at cell–cell and cell–matrix adhesions
Vinculin phosphorylation on residue Y822 is necessary for cell stiffening in response to tension on cadherins but not integrins.Cells experience mechanical forces throughout their lifetimes. Vinculin is critical for transmitting these forces, yet how it achieves its distinct functions at cell–cell and cell–matrix adhesions remains unanswered. Here, we show vinculin is phosphorylated at Y822 in cell–cell, but not cell–matrix, adhesions. Phosphorylation at Y822 was elevated when forces were applied to E-cadherin and was required for vinculin to integrate into the cadherin complex. The mutation Y822F ablated these activities and prevented cells from stiffening in response to forces on E-cadherin. In contrast, Y822 phosphorylation was not required for vinculin functions in cell–matrix adhesions, including integrin-induced cell stiffening. Finally, forces applied to E-cadherin activated Abelson (Abl) tyrosine kinase to phosphorylate vinculin; Abl inhibition mimicked the loss of vinculin phosphorylation. These data reveal an unexpected regulatory mechanism in which vinculin Y822 phosphorylation determines whether cadherins transmit force and provides a paradigm for how a shared component of adhesions can produce biologically distinct functions
Estimating Hispanic-White Wage Gaps Among Women: The Importance of Controlling for Cost of Living
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