Detection of Anammox Bacteria from Forest soils of Different Thinning Intensity

Poster Sessio

Enrichment and Characterization of an Ammonia-Oxidizing Archaeon of Moderate Thermophilic Taumarchaeotal Group I.1a from Cattle Manure Compost

Poster Presentatio

Relationship between Nitrogen Concentration and Ammonia Oxidizing Microbes in the Japanese Cedar Forest Soils under Different Managements

Poster Sessio

Isolation and Characterization of a Thermotolerant Ammonia-Oxidizing Bacterium Nitrosomonas sp. JPCCT2 from a Thermal Power Station

A thermotolerant ammonia-oxidizing bacterium strain JPCCT2 was isolated from activated sludge in a thermal power station. Cells of JPCCT2 are short non-motile rods or ellipsoidal. Molecular phylogenetic analysis of 16S rRNA gene sequences demonstrated that JPCCT2 belongs to the genus Nitrosomonas with the highest similarity to Nitrosomonas nitrosa Nm90 (100%), Nitrosomonas sp. Nm148 (99.7%), and Nitrosomonas communis Nm2 (97.7%). However, G+C content of JPCCT2 DNA was 49.1 mol % and clearly different from N. nitrosa Nm90, 47.9%. JPCCT2 was capable of growing at temperatures up to 48°C, while N. nitrosa Nm90 and N. communis Nm2 could not grow at 42°C. Moreover, JPCCT2 grew similarly at concentrations of carbonate 0 and 5 gL−1. This is the first report that Nitrosomonas bacterium is capable of growing at temperatures higher than 37°C. Key words: Nitrosomonas, thermotolerant ammonia-oxidizing bacterium, activated sludge Chemolithoautotrophic ammonia-oxidizing bacteria (AOB), which convert ammonium to nitrite, play an important role in the global cycling of nitrogen (19, 22). Isolation of AOB was first reported in 1890 (2, 28), and since then a con-siderable number of AOB within the Betaproteobacteria and Gammaproteobacteria have been obtained from various envi