1 research outputs found
Development and Application of Ligand-Exchange Reaction Method for the Determination of Clonazepam
Purpose: This paper presents an improved kinetic-spectrophotometric
procedure for determining clonazepam (CZP) in pharmaceutical
formulations and human serum. Methods: The method is based on
ligand-exchange reaction. The reaction was followed
spectrophotometrically by measuring the rate of change of absorbance at
425 nm in ethanolic sodium hydroxide solution.3 Results: The optimum
operating conditions for reagent concentrations and temperature were
established. Linear calibration curve was obtained in the range of 0.32
- 4.10 μg mL-1. The optimized conditions yielded a theoretical
detection limit of 0.24 μg mL-1 based on the 3.3So criterion,
where S0 is standard deviation of the calibration line. The
interference of certain drugs, foreign ions and amino acids on the
reaction rate were studied in order to assess the selectivity of the
method. Conclusion: The developed method is sensitive, accurate and
reproducible and could be used for routine anlysis of clonazepam in
pharmaceutical preparations and serum samples