Antagonistic and plant growth promoting properties of actinomycetes from rhizosphere Deschampsia antarctica È. Desv. (Galindez Island, Antarctica)

Aim. The isolation of actinomycetes from Deschampsia antarctica É. Desv. (Galindez Island, Antarctica) rhisosphere and determine their ability to produce compounds with antimicrobial and plant growth promotional properties.Мета. Виділення актиноміцетів з ризосфери Deschampsia antarctica É. Desv. популяцій антарктичного острова Галіндез, оцінка їхньої здатності пригнічувати фітопатогенні мікроорганізми і продукувати фітостимулювальні сполуки

Design, development and application of whole cell based antibiotic specific biosensor

Synthetic biology techniques hold great promise for optimising the production of natural products by microorganisms. However, evaluating the phenotype of a modified bacterium represents a major bottleneck to the engineering cycle particularly for antibiotic producing actinobacteria strains, which grow slowly and are challenging to genetically manipulate. Here, we report the generation and application of antibiotic specific whole cell biosensor derived from TetR transcriptional repressor for use in identifying and optimising antibiotic producers. The constructed biosensor was successfully used to improve production of polyketide antibiotic pamamycin. However, an initial biosensor based on native genetic elements had inadequate dynamic and operating ranges. To overcome these limitations, we fine tuned biosensor performance through alterations of the promoter and operator of output module and the ligand affinity of transcription factor module, which enabled us to deduce recommendations for building and application of actinobacterial biosensor