8 research outputs found
Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
Neolamarckia cadamba or commonly known as kelampayan has been selected as one of the important plantation
tree species in Malaysia. Thus, the molecular characterization of this indigenous tropical tree species is needed to
maintain its high quality. Inter simple sequence repeats (ISSR) markers were used in this study to determine the
genetic diversity and relatedness of N. cadamba in two planted forests and six natural forests in Sarawak. Three
ISSR primers had generated atotal of 239 loci, of which 32.6% - 59.4% of the loci were polymorphic among236N.
cadamba treesin eight populations. The mean Shannon’s diversity index (I)ranged from 0.1399 to 0.2354. The
coefficient of population differentiation was low for planted forests (Gst = 0.0871) and natural forests (Gst =
0.2013`). Both UPGMA dendrogram and NJ-tree generated by ISSR markers had divided natural forests and
planted forests into two distinct clusters. Natural forests were grouped in one cluster while planted forests were
grouped in another cluster. This study shows that N. cadamba trees are closely related within its own population
and its designated forest type. In future, several specific loci can be sequenced and developed into SCAR (sequence
characterized amplification region) markers for tree improvement and conservation programme of N. cadamba
Recommended from our members
An innovative integral field unit upgrade with 3D-printed micro-lenses for the RHEA at Subaru
In the new era of Extremely Large Telescopes (ELTs) currently under construction, challenging requirements drive spectrograph designs towards techniques that efficiently use a facility's light collection power. Operating in the single-mode (SM) regime, close to the diffraction limit, reduces the footprint of the instrument compared to a conventional high-resolving power spectrograph. The custom built injection fiber system with 3D-printed microlenses on top of it for the replicable high-resolution exoplanet and asteroseismology spectrograph (RHEA) at Subaru in combination with extreme adaptive optics of SCExAO, proved its high efficiency in a lab environment, manifesting up to ∼77% of the theoretical predicted performance. © 2020 SPIE.Immediate accessThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]