240 research outputs found
Cytochrome P450 1A1 genetic polymorphisms and risk of hepatocellular carcinoma among chronic hepatitis B carriers
5-s Rna. Biosynthesis and association to immature ribosomal particles in the nucleus of lymphocytic mouse leukemia cells, l5178y.
Evidence for the existence of several molecular species in the \u2745s fraction\u27 of mammalian ribosomal precursor rna.
Effects of valine deprivation on the biosynthesis of ribosomal and messenger rna in a mammalian cell line (l5178y).
Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases.
We have constructed vectors from bacteriophage lambda and from plasmid pBR322 having a single EcoRI restriction site which is immediately downstream from the lac UV5 promotor. Each vector allows the fusion of a cloned gene to the lac Z gene in a different phase relative to the translation initiation codon of the lac Z gene. These vectors were constructed through modification of the initial EcoRI restriction site by S1 endonuclease treatment and then addition of octadeoxyribonucleotides (EcoRI linkers), which shifted the restriction site by 2 or 4 nucleotides. Used in combination these vectors should allow translation of a cloned gene in any one of the three coding phases. The bacteriophages vectors are certified as B2 (EK2) safety level vectors by the French "recombinaison génétique in vitro" committee (D.G.R.S.T.)
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