1 research outputs found
Biosensing Test-Bed Using Electrochemically Deposited Reduced Graphene Oxide
The
development of an efficient test-bed for biosensors requires stable
surfaces, capable of interacting with the functional groups present
in bioentities. This work demonstrates the formation of highly stable
electrochemically reduced graphene oxide (ERGO) thin films reproducibly
on indium tin oxide (ITO)-coated glass substrates using a reliable
technique through 60 s chronoamperometric reduction of a colloidal
suspension maintained at neutral pH containing graphene oxide in deionized
water. Structural optimization and biocompatible interactions of the
resulting closely packed and uniformly distributed ERGO flakes on
ITO surfaces (ERGO/ITO) are characterized using various microscopic
and spectroscopic tools. Lipase enzyme is immobilized on the ERGO
surface in the presence of ethyl-3-[3-(dimethylamino)Âpropyl]Âcarbodimide
and <i>N</i>-hydroxysuccinimide for the detection of triglyceride
in a tributyrin (TBN) solution. The ERGO/ITO surfaces prepared using
the current technique indicate the noticeable detection of TBN, a
source of triglycerides, at a sensitivity of 37 pA mg dL<sup>–1</sup> cm<sup>–2</sup> in the linear range from 50 to 300 mg dL<sup>–1</sup> with a response time of 12 s. The low apparent Michaelies–Menten
constant of 0.28 mM suggests high enzyme affinity to TBN. The currently
developed fast, simple, highly reproducible, and reliable technique
for the formation of an ERGO electrode could be routinely utilized
as a test bed for the detection of clinically active bioentities