Additional file 1: of Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

Table S1. List of primers used in this study. (PDF 63 kb

Additional file 4: of Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

Figure S3. (A). Relative mRNA expression levels of FABP4, FABP5 and CD36 in breast cancer cell lines. (B). Tumor cells were transfected with FABP5-targeting siRNA, and cells were harvested at day 6 after transfection for western blotting. (PDF 362 kb

Additional file 3: of Utilization of adipocyte-derived lipids and enhanced intracellular trafficking of fatty acids contribute to breast cancer progression

Figure S2. Representative immunohistochemical staining of ATGL in normal breast and tumor tissues. (PDF 2736 kb

Additional file 2: of TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells

Figure S2. The methylation and expression of TET1, DACTs, WNTs in HNSC. Data from TCGA ( http://methhc.mbc.nctu.edu.tw/php/index.php ). (TIF 82 kb

<i>ZNF545</i> is frequently methylated in primary breast tumors.

<p>(A) Methylation of <i>ZNF545</i> by MSP in normal breast tissues. (B) Representative analysis of methylation of the <i>ZNF545</i> promoter in breast tumor tissues. M, methylated; U, unmethylated.</p

Methylation status of the <i>UCHL1</i> promoter in primary breast tumors.

<p>Note: BrCa, breast cancer; BA, breast cancer adjacent tissues; BNP, breast normal tissues.</p

Reduced expression of ZNF545 in breast cancer.

<p>Data extracted from cancer microdatabase Oncomine: <a href="https://www.oncomine.org/" target="_blank">https://www.oncomine.org/</a>. ICBC: Intraductal Cribriform Breast Adenocarcinoma; MBC: Male Breast Carcinoma; IC: Invasive Breast Carcinoma; IDC: invasive ductal breast carcinoma; ILC: invasive lobular breast carcinoma; LC: lobular breast carcinoma.</p><p>Reduced expression of ZNF545 in breast cancer.</p

Relationship between ER/PR/HER2 status and ZNF545 expression in paired breast cancer tissues.

<p>Relationship between ER/PR/HER2 status and ZNF545 expression in paired breast cancer tissues.</p

Effect of UCHL1 on cell cycle distribution of MB231 cells.

<p>(A) Representative cell cycle analysis. (B) Summarized flow cytometry data. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029783#s2" target="_blank">Results</a> are represented as average±S.D and are based on three independent experiments. Statistical significance was determined using Student's t-test (p<0.001).</p

Promoter methylation of <i>UCHL1</i> in primary breast tumors.

<p>(A, B, C) Representative analysis of <i>UCHL1</i> methylation by MSP in normal breast tissues, breast tumor adjacent tissues and primary tumors. M, methylated; U, unmethylated. (D) Detailed BGS analysis of <i>UCHL1</i> promoter in primary tumor and normal breast tissue. Circles, single CpG sites analyzed; row of circles, individual promoter allele cloned, randomly selected and sequenced; Filled circle, methylated; empty circle, unmethylated.</p