Additional file 1 of Association between nutritional status scores and the 30-day mortality in patients with acute kidney injury: an analysis of MIMIC-III database

Supplementary Material

Additional file 1 of Association between white blood cell count to hemoglobin ratio and risk of in-hospital mortality in patients with lung cancer

Additional file 1: Supplementary Table 1. Sensitivity analysis before and after interpolatio

Self-Assembly of Catecholic Macroinitiator on Various Substrates and Surface-Initiated Polymerization

A catechol-containing macroinitiator has been designed for the surface-initiated atom transfer radical polymerization (SI-ATRP) from various substrates at ambient temperature. Temperature-sensitive poly­(<i>N</i>-isopropyl acrylamide) (PNIPAM) brushes were successfully grafted from a range of substrates surfaces, including metals and polyimides, via SI-ATRP using the resulting macroinitiator, which were characterized by X-ray photoelectron spectroscopy (XPS), water contact angle measurements, and atomic force microscopy (AFM). Effects of the temperature response behavior of PNIPAM brushes on the water contact angles and the impedance of the modified surfaces were also exhibited. The self-assembled film of macroinitiator and the resulting polymer brushes were both stable to soaking of basic solvents, and the brushes did not show any exfoliation or delamination even after 2 h of ultrasonic test. The advantages of the macroinitiator in strong interactions with surfaces and high stability and convenience make it possible to modify the native materials with polymer brushes in a convenient and nondestructive way. Importantly, the macroinitiator is compatible with microcontact printing, and patterned polymer brushes on Ti plate were demonstrated by microcontact printing of BrDOPAMA and the following SI-ATRP

Efficient Removal of Methyl Orange and Alizarin Red S from pH-Unregulated Aqueous Solution by the Catechol–Amine Resin Composite Using Hydrocellulose as Precursor

We report a novel composite absorbent prepared by the simple method that catechol-amine resin coats the hydrocellulose based on the adhesion property like polydopamine. The composite which contains many chelating groups on its surface was characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), etc. The obtained adsorbents were investigated to remove Methyl Orange (MO) and Alizarin Red S (AR) from pH-unregulated aqueous system by batch experiments, including the affected factors of adsorbent dosage, contact time, initial concentration, and temperature. Results showed the adsorption processes belonged to the chemisorption and exhibited a spontaneous and endothermic nature. Besides, the removal performances fitted with the Langmuir isotherm model and pseudo-second order kinetic model very well. The maximum adsorption amounts of MO and AR were 189.39 and 284.09 mg/g at 303 K, respectively. The difference about adsorption amounts may be caused by the strong effect of π–π conjugation and hydrogen bonding between adsorbent and AR. Furthermore, the adsorption processes exhibited a spontaneous and endothermic nature. The recycling test indicated that the adsorbent stayed stable for the removal of both dyes by desorbed three times. Accordingly, the adsorbent with high adsorption capacity and rapid removal rate should be a promising material for the removal of anionic dyes from sewage

Facile Preparation of Tannic Acid–Poly(vinyl alcohol)/Sodium Alginate Hydrogel Beads for Methylene Blue Removal from Simulated Solution

A novel hydrogel bead [tannic acid (TA)–poly­(vinyl alcohol) (PVA)/sodium alginate (SA)] with high strength prepared by biocompatible PVA, TA, and biocompatible SA via an instantaneous gelation method was applied to remove methylene blue (MB) from aqueous solution. The obtained TA–PVA/SA hydrogel beads were fully characterized by thermogravimetric analysis, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and so on. The adsorption performances of TA–PVA/SA hydrogel beads for MB were investigated by changing the factors of TA content, initial concentration, pH, adsorbent dosage, contact time, and temperature systematically. The maximum capacity of TA–PVA/SA hydrogel beads for MB removal was obtained to be 147.06 mg/g at 30 °C, whose capability was better than that without TA. After fitting the adsorbed data, it was basically consistent with the Langmuir isotherm and pseudo-second-order kinetic model. Thermodynamic studies indicated that MB removal was spontaneous and exothermic in nature. It is concluded that the low-cost TA–PVA/SA hydrogel beads as an easily recoverable adsorbent have a great potential on the removal of hazardous dyes from wastewater

Data_Sheet_1_Global research trends in in-stent neoatherosclerosis: A CiteSpace-based visual analysis.docx

BackgroundRecent studies have shown that in-stent neoatherosclerosis (ISNA/NA) is an important cause of late stent failure. A comprehensive understanding of the current state of research in this field will facilitate the analysis of its development trends and hot frontiers. However, no bibliometric correlation has been reported yet. Here, we analyze the relevant literature since the emergence of the concept and provide valuable insights.MethodsPublications were collected from the Web of Science Core Collection (WoSCC) and PubMed. Microsoft Excel, SPSS and CiteSpace were used to analyze and present the data.ResultsA total of 498 articles were collected, with Japan and Cardiovasc Res Fdn being the main publishing forces in all country/region and institutions. J AM COLL CARDIOL is the journal with the most published and co-cited articles. According to co-citation analysis, optical coherence tomography, thrombosis, implantation, restenosis, drug-eluting stent, and bare metal stent have become more and more popular recently.ConclusionISNA is a niche and emerging field. How to reduce the incidence of ISNA and improve the late patency rate of coronary stents may remain a hot spot for future research. The pathogenesis of ISNA also needs to be explored in more depth.</p

All-in-One Compact Architecture toward Wearable All-Solid-State, High-Volumetric-Energy-Density Supercapacitors

High-performance flexible energy storage devices are an important prerequisite to the utilization of various advanced wearable electronics, such as healthcare sensors and smart textiles. In this work, we design a wearable all-solid-state, all-in-one asymmetric supercapacitor by integrating current collectors, a separator, and negative and positive electrodes into a thin, flexible, and porous polyamide nanofiber film. The positive and negative electrodes are, respectively, electrodeposited onto each side of the carbon nanotube-modified porous polyamide nanofiber film to form the integrated and compact asymmetric cell. The all-in-one thin-film asymmetric supercapacitor is binder-, additive-, and metal current collector-free, which can effectively decrease the cost, simplify the assembly procedures, and increase the energy density. The assembled flexible all-in-one asymmetric supercapacitor with a compact structure shows high gravimetric and volumetric specific capacitances of 70 F g^–1 and 3.1 F cm^–3 under a current density of 0.5 A g^–1 in a neutral polyvinyl alcohol/LiCl gel electrolyte, respectively. Additionally, the all-in-one asymmetric cell displays a favorable volumetric energy density of 1.1 W h L^–3, which is among the highest compared with other reported flexible solid-state supercapacitors. Notably, multiple cell units can be integrated in one piece of polyamide nanofiber film and connected in series to satisfy the need of high output voltage

Massively Parallel Patterning of Complex 2D and 3D Functional Polymer Brushes by Polymer Pen Lithography

We report the first demonstration of centimeter-area serial patterning of complex 2D and 3D functional polymer brushes by high-throughput polymer pen lithography. Arbitrary 2D and 3D structures of poly­(glycidyl methacrylate) (PGMA) brushes are fabricated over areas as large as 2 cm × 1 cm, with a remarkable throughput being 3 orders of magnitudes higher than the state-of-the-arts. Patterned PGMA brushes are further employed as resist for fabricating Au micro/nanostructures and hard molds for the subsequent replica molding of soft stamps. On the other hand, these 2D and 3D PGMA brushes are also utilized as robust and versatile platforms for the immobilization of bioactive molecules to form 2D and 3D patterned DNA oligonucleotide and protein chips. Therefore, this low-cost, yet high-throughput “bench-top” serial fabrication method can be readily applied to a wide range of fields including micro/nanofabrication, optics and electronics, smart surfaces, and biorelated studies

Table_2_Identification of bi-allelic KIF9 loss-of-function variants contributing to asthenospermia and male infertility in two Chinese families.xlsx

IntroductionAsthenozoospermia (AZS) is a leading cause of male infertility, affecting an estimated 18% of infertile patients. Kinesin proteins function as molecular motors capable of moving along microtubules. The highly conserved kinesin family member 9 (KIF9) localizes to the central microtubule pair in the flagella of Chlamydomonas cells. The loss of KIF9 expression in mice has been linked to AZS phenotypes.MethodsVariant screening was performed by whole exome sequencing from 92 Chinese infertile patients with AZS. Western blot was used to was used for analyzing of candidate proteins expression. Patients’ sperm samples were stained with immunofluorescent to visualise proteins localization and were visualised by transmission electron microscopy (TEM) to determine axoneme structures. Co-immunoprecipitation assay was used to verify the binding proteins of KIF9. In vitro fertilization (IVF) was used to evaluate the efficiency of clinical treatment.ResultsBi-allelic KIF9 loss-of-function variants were identified in two unrelated Chinese males exhibiting atypical sperm motility phenotypes. Both of these men exhibited typical AZS and suffered from infertility together with the complete absence of KIF9 expression. In contrast to these KIF9-deficient patients, positive KIF9 staining was evident throughout the flagella of sperm from normal control individuals. KIF9 was able to interact with the microtubule central pair (CP) component hydrocephalus-inducing protein homolog (HYDIN) in human samples. And KIF9 was undetectable in spermatozoa harboring CP deletions. The morphologicy of KIF9-deficient spermatozoa appeared normal under gross examination and TEM. Like in mice, in vitro fertilization was sufficient to overcome the fertility issues for these two patientsDiscussionThese findings indicate that KIF9 associates with the central microtubules in human sperm and that it functions to specifically regulate flagellar swinging. Overall, these results offer greater insight into the biological functions of KIF9 in the assembly of the human flagella and its role in male fertility.</p

Table_1_Identification of bi-allelic KIF9 loss-of-function variants contributing to asthenospermia and male infertility in two Chinese families.xlsx

IntroductionAsthenozoospermia (AZS) is a leading cause of male infertility, affecting an estimated 18% of infertile patients. Kinesin proteins function as molecular motors capable of moving along microtubules. The highly conserved kinesin family member 9 (KIF9) localizes to the central microtubule pair in the flagella of Chlamydomonas cells. The loss of KIF9 expression in mice has been linked to AZS phenotypes.MethodsVariant screening was performed by whole exome sequencing from 92 Chinese infertile patients with AZS. Western blot was used to was used for analyzing of candidate proteins expression. Patients’ sperm samples were stained with immunofluorescent to visualise proteins localization and were visualised by transmission electron microscopy (TEM) to determine axoneme structures. Co-immunoprecipitation assay was used to verify the binding proteins of KIF9. In vitro fertilization (IVF) was used to evaluate the efficiency of clinical treatment.ResultsBi-allelic KIF9 loss-of-function variants were identified in two unrelated Chinese males exhibiting atypical sperm motility phenotypes. Both of these men exhibited typical AZS and suffered from infertility together with the complete absence of KIF9 expression. In contrast to these KIF9-deficient patients, positive KIF9 staining was evident throughout the flagella of sperm from normal control individuals. KIF9 was able to interact with the microtubule central pair (CP) component hydrocephalus-inducing protein homolog (HYDIN) in human samples. And KIF9 was undetectable in spermatozoa harboring CP deletions. The morphologicy of KIF9-deficient spermatozoa appeared normal under gross examination and TEM. Like in mice, in vitro fertilization was sufficient to overcome the fertility issues for these two patientsDiscussionThese findings indicate that KIF9 associates with the central microtubules in human sperm and that it functions to specifically regulate flagellar swinging. Overall, these results offer greater insight into the biological functions of KIF9 in the assembly of the human flagella and its role in male fertility.</p