Additional file 2: Figure S2. of WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism

Effect of WWL70 in recombinant COX assay. Ovine recombinant COX-1 (A) or human recombinant COX-2 (B) was pre-incubated in 0.1 M Tris-HCl (pH 8.0) and EDTA (1 mM) buffer with WWL70 (10 μM), SC-560 (0.33 μM), or Dup-697 (0.3 μM) for 5 min at 27 °C. Then, 10 μM of AA was added and incubated for 1 min at 27 °C. The reaction mixture was applied for prostaglandins EIA to measure total amount of prostaglandins. (PPTX 42 kb

Additional file 1: Figure S1. of WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism

Conversion of 2-AG to AA in membrane fraction. Membrane fraction from BV2 cells was incubated with 100 μM of 2-AG-d8 for 0, 1, or 5 min at 37 °C. AA-d8 extracted with acetonitrile was applied to LC-MS/MS. Mass spectrograms for the MRM transition from m/z 312 to m/z 93 is shown at three time points. Transition peaks of each time point are shown in inset represented as mean ± SD (n = 3). (PPTX 53 kb