3,323 research outputs found

    Photothermal nanoblade for patterned cell membrane cutting.

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    We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells

    Image patterned molecular delivery into live cells using gold particle coated substrates.

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    An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated

    Whether Adoption Drivers Differ between Click-and-mortar and Pure-play E-payment Services?

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    How could traditional financial institutions attract online users? Should they mimic their online counter-parts, or compete based on an existing offline business? This research compares different effects of the adoption drivers between pure-play and click-and-mortar e-payment services based on a trust-based Valence Framework. Use intention is proposed to be affected by perceived benefit, perceived risk, and trust, which is in turn affected by familiarity, reputation and security protection. 276 subjects’ responses about Quick Pay (a pure online third-party payment) and Union Pay (an e-payment service offered by a traditional financial institution) were collected. The data analysis reveals: (1) the pure-play e-payment performs much better than the click-and-mortar e-payment except for information risk and property risk; (2) all the path coefficients are significant except the link between perceived risk and use intention for Union Pay; (3) most of the path coefficients for pure-play e-payment service are stronger than those of click-and-mortar e-payment service, except for the links between perceived benefit and intention, trust and perceived risk, and familiarity and trust. These differences can be attributed to different resource endowments owned by service providers. The results suggest that pure-play and click-andmortar e-payment should have different focuses when promoting their services

    Influence Maximization based on Simplicial Contagion Model in Hypergraph

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    In recent years, the issue of node centrality has been actively and extensively explored due to its applications in product recommendations, opinion propagation, disease spread, and other areas involving maximizing node influence. This paper focuses on the problem of influence maximization on the Simplicial Contagion Model, using the susceptible-infectedrecovered (SIR) model as an example. To find practical solutions to this optimization problem, we have developed a theoretical framework based on message passing processes and conducted stability analysis of equilibrium solutions for the self-consistent equations. Furthermore, we introduce a metric called collective influence and propose an adaptive algorithm, known as the Collective Influence Adaptive (CIA), to identify influential propagators in the spreading process. This method has been validated on both synthetic hypergraphs and real hypergraphs, outperforming other competing heuristic methods.Comment: 19 pages,16 figure

    ONLINE USER INTENTION TO SELECT A SHARED ACCOUNT OPTION ON MULTI-SERVICE PLATFORMS

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    Online users are free to register an account on a website for the purpose of enjoying various online services. More and more multi-service platforms are being developed. Users have the option to either create a new account or simply share their original account information, e.g., Facebook, to complete registration. However, when using the original account information on a new platform, online users are possibly at risk disclosing their personal information to platform vendors. Therefore, this study\u27s purpose is to explore online user intention toward using shared accounts on another new multi-service platform. Individual aspects of perceived risk and perceived benefits for using shared accounts are examined through an online survey which was validated by MIS experts and passed a pilot test. The preliminary results of this study show that registration efficiency and perceived platform trust level have significant positive impacts on user intention, which further impacts their actual behavior related to using a shared account while user privacy concerns and the perceived security level of the platform have a significant negative impact on their use intention. Discussion is provided along with other data sources arguing the non-significant effects of perceived enjoyment and perceived usefulness on online user intention
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