Additional file 1: of Identification of non-essential loci within the Meleagrid herpesvirus 1 genome

Supplemental Table S1. Details of transposon gene interruptions into the MeHV-1 infectious clone pMeHV1-C18. Supplemental Table S2: Oligonucleotides used in this study. Supplemental Figure S1: Primary chicken embryo fibroblasts (CEFs) were used as a negative control. (PDF 121 kb

Additional file 2: Table S1. of The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1

Oligonucleotides used for ORF transcription studies of the unique hypothetical MaHV-1 ORFs (PW1 to PW6) and the host housekeeping gene GAPDH. (DOC 37 kb

Additional file 1: Figure S1. of The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1

Relative transcript levels for the unique hypothetical MaHV-1 ORFs PW1 to PW6 at 4 h (grey bars) and 12 h (black bars) post infection (hpi) in wallaby fibroblast cells. Expression was normalised to the host housekeeping gene, GAPDH, and analysed by calculating mean normalised expression values. No viral transcripts were detected in uninfected cells. Error bars indicate standard deviation of three biological replicates. (TIF 8005 kb

Phylogenetic relationship between macropodid herpesvirus 1 (MaHV1) and other viruses from the Alphaherpesvirinae subfamily.

<div>The relationship between macropodid herpesvirus 1 (MaHV1) and other viruses from the Alphaherpesvirinae subfamily. Neighbour-joining distance trees were generated using the translated protein sequences of conserved herpesvirus ORFs: a glycoprotein B, gB (UL27); b DNA polymerase, DPOL (UL30); and c glycoprotein D, gD (US6). Bootstrap values (10,000 replicates) are shown for each branch, with Gallid herpesvirus 2 (GaHV2) as an outgroup. The abbreviations and GenBank accession details are: macropodid herpesvirus 2 (MaHV2) [GenBank:AAD11961, GenBank:AAL13143, gB and gD], macropodid herpesvirus 4 (MaHV4) [GenBank:AGC54689, gB], macacine herpesvirus 1 (HBV) [GenBank:AAA85652, GenBank:NP_851890, GenBank:AAB24129, gB, DPOL and gD], fruit bat herpesvirus 1 (FbHV1) [GenBank:YP_009042089, GenBank:YP_009042092, GenBank:YP_009042126, gB, DPOL and gD], gallid herpesvirus 1 (GaHV1) [GenBank:AEB97319 , GenBank:AEB97322, GenBank:AEB97368, gB, DPOL and gD], gallid herpesvirus 2 (GaHV2) [GenBank:CAA63039, GenBank:YP_001033959, GenBank:AAA64967, gB, DPOL and gD], human herpesvirus 1 (HHV1) [GenBank:NP_044629, GenBank:NP_044632, GenBank:CAA32283, gB, DPOL and gD], human herpesvirus 2 (HHV2) [GenBank:NP_044497, GenBank:NP_044500, GenBank:AAB60553, gB, DPOL and gD], human herpesvirus 3 (HHV3) [GenBank:NP_040154.2, GenBank:NP_040151, gB and DPOL], saimiriine herpesvirus 1 (SaHV1) [GenBank:YP_003933812, GenBank:YP_003933809, GenBank:ADO13836, gB, DPOL and gD], bovine herpesvirus 1 (BoHV1) [GenBank:AAA46013, GenBank:NP_045328, GenBank:CAA80604, gB, DPOL and gD], bovine herpesvirus 2 (BoHV2) [GenBank:P12641.2, GenBank:AAD55134:, gB and DPOL], bovine herpesvirus 5 (BoHV5) [GenBank:YP_003662497, GenBank:YP_003662494, GenBank:AAA67359, gB, DPOL and gD], canine herpesvirus 1 (CHV) [GenBank:AAK51052, GenBank:AAX47050, GenBank:AAB67058, gB, DPOL and gD], felid herpesvirus 1 (FeHV1) [GenBank:AAB28559, GenBank:YP_003331549, GenBank:BAA06442, gB, DPOL and gD], equine herpesvirus 1 (EHV1) [GenBank:Q6DLH8, GenBank:YP_053075, GenBank:AAA46073, gB, DPOL and gD], equine herpesvirus 4 (EHV4) [GenBank:NP_045250, GenBank:NP_045247, GenBank:NP_045289, gB, DPOL and gD], suid herpesvirus 1 (PRV) [GenBank:YP_068330, GenBank:YP_068333, GenBank:AAC36717, gB, DPOL and gD].</div