Human-on-a-Chip

A high level video explaining the idea behind developing a human-on-a-chip where multiple organ-on-a-chip models interact to mimic human physiology for the purpose of drug testing

PrestoBlue for Quantifying Rat Islet Viability and Count

Rat pancreatic islets obtained from the Joslin Diabetes Center were size filtered to 70-140 um diameter range. Islets were handpicked and placed in a 24-well culture plate. Each well contained 880 uL of RPMI (with 0.125% BSA). Islets were collected in a total volume of 200 uL and added to the well. 120 uL of PrestoBlue (Thermo) was added to each well and incubated for either 1 or 2 hours. 100 uL was collected and the fluorescence signal was measured in a clear bottom black-walled 96 well plate. N = 4, error bars represent standard deviation. <div><br></div><div>All work was conducted at the Massachusetts Institute of Technology, Cambridge, MA.</div

MICCS

Multipurpose Integrated Cell Culture Syste