Hop to It! The Relationship Between Hop Tests and The Anterior Cruciate Ligament – Return to Sport Index After Anterior Cruciate Ligament Reconstruction in NCAA Division 1 Collegiate Athletes

# Background Outcomes after anterior cruciate ligament reconstruction (ACLR) may not be optimal, with poor physical and psychological function potentially affecting return to sport (RTS) ability. Understanding the relationship between commonly used hop tests and the Anterior Cruciate Ligament -- Return to Sport Index (ACL-RSI) may improve rehabilitation strategies and optimize patient outcomes. # Hypothesis/Purpose The purpose of this study was to examine the relationship between ACL-RSI scores and limb symmetry index (LSI) for the single hop for distance (SHD), triple hop for distance (THD), crossover hop for distance (CHD), timed 6-meter hop (T6H), and single leg vertical hop (SLVH) in a cohort of National Collegiate Athletic Association (NCAA) Division 1 collegiate athletes after ACLR. The hypothesis was that SLVH LSI would be more highly correlated with ACL-RSI score than all horizontal hop tests. # Study design Cross-Sectional Study # Methods Twenty-one National Collegiate Athletic Association (NCAA) Division 1 collegiate athletes (7 males, 14 females) at 6.62 ± 1.69 months after ACLR were included in this retrospective study. Primary outcomes were ACL-RSI score and LSI for SHD, THD, CHD, T6H, and SLVH. The relationship between ACL-RSI scores and performance on hop tests (LSIs) was evaluated using correlation analysis and step-wise linear regression (p ≤ 0.05). # Results There were significant correlations found when comparing ACL-RSI and the LSI for SHD (r~s~ = 0.704, p \< 0.001), THD (r~s~ = 0.617, p = 0.003), CHD (r~s~ = 0.580, p = 0.006), and SLVH (r~s~ = 0.582, p = 0.006). The CHD explained 66% (R^2^ value of 0.660) of the variance in the ACL-RSI, while the other hop tests did not add to the predictive model. # Conclusions Physical function has the capacity to influence psychological status after ACLR. Clinicians should recognize that SLVH, SHD, THD, and CHD are correlated with ACL-RSI and improvements in physical function during rehabilitation may improve psychological status and optimize RTS after ACLR. # Level of evidence Level

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Tumor-targeted, systemic delivery of therapeutic viral vectors using hitchhiking on antigen-specific T cells

Antigen-specific T cells circulate freely and accumulate specifically at sites of antigen expression. To enhance the survival and targeting of systemically delivered viral vectors, we exploited the observation that retroviral particles adhere nonspecifically, or 'hitchhike,' to the surface of T cells. Adoptive transfer of antigen-specific T cells, loaded with viruses encoding interleukin (IL)-12 or Herpes Simplex Virus thymidine kinase (HSVtk), cured established metastatic disease where adoptive T-cell transfer alone was not effective. Productive hand off correlated with local heparanase expression either from malignant tumor cells and/or as a result of T-cell activation by antigen, providing high levels of selectivity for viral transfer to metastatic tumors in vivo. Protection, concentration and targeting of viruses by adsorption to cell carriers represent a new technique for systemic delivery of vectors, in fully immunocompetent hosts, for a variety of diseases in which delivery of genes may be therapeutically beneficial

Evidence for a spike in mantle carbon outgassing during the Ediacaran period

© 2017 The Author(s). Long-term cycles in Earth\u27s climate are thought to be primarily controlled by changes in atmospheric CO2 concentrations. Changes in carbon emissions from volcanic activity can create an imbalance in the carbon cycle. Large-scale changes in volcanic activity have been inferred from proxies such as the age abundance of detrital zircons, but the magnitude of carbon emissions depends on the style of volcanism as well as the amount. Here we analyse U-Pb age and trace element data of detrital zircons from Antarctica and compare the results with the global rock record. We identify a spike in CO2-rich carbonatite and alkaline magmatism during the Ediacaran period. Before the Ediacaran, secular cooling of the mantle and the advent of cooler subduction regimes promoted the sequestration of carbon derived from decarbonation of subducting oceanic slabs in the mantle. We infer that subsequent magmatism led to the extensive release of carbon that may at least in part be recorded in the Shuram-Wonoka carbon isotope excursion. We therefore suggest that this pulse of alkaline volcanism reflects a profound reorganization of the Neoproterozoic deep and surface carbon cycles and promoted planetary warming before the Cambrian radiation