MnTBAP treatment reduces caloric intake in mice fed a HFD.

<p><b>(A)</b> Caloric intake was assessed at various time points of MnTBAP treatment in mice previously fed a LFD or HFD for 5 months. <b>(B)</b> Correlation between the change in body weight and the change in caloric intake from pre-treatment to post-treatment. *, Denotes statistically significant difference from HFD-Vehicle. ⁺, Denotes statistically significant difference from respective LFD mice. P-value for post-hoc analysis for LFD-Vehicle vs. HFD-Vehicle mice: Day 9, P = 0.057; Day 17, P = 0.085. N = 6–8 mice per group.</p

The HO-1 inhibitor SnMP does not block MnTBAP’s ability to reduce body weight, adipose tissue mass, and caloric intake in mice fed HFD.

<p>Mice were fed a LFD or HFD for 5 months and then treated with MnTBAP (10 mg/kg) alone or in combination with SnMP (20 mg/Kg) daily for 5 weeks. <b>(A)</b> Fed-state body weights at different treatment durations. <b>(B)</b> Epididymal white adipose tissue (EWAT) mass. (C) Caloric intake was assessed at various time points of MnTBAP or MnTBAP+SnMP treatment in mice previously fed a LFD or HFD for 5 months. *, Denotes statistically significant difference from LFD vehicle-treated mice. ⁺, Denotes statistically significant difference from respective LFD mice. N = 5–6 mice per group.</p

MnTBAP treatment improves insulin-assisted glucose tolerance (IAGT) in mice fed a HFD.

<p>Mice were simultaneously injected with 2.0 U/Kg insulin and 2.0 g/Kg glucose and blood glucose values were assessed at baseline and 20, 40, and 60 min following the injection (Panels A) and the area under the IAGT curve was calculated (Panels B). *, Denotes statistically significant difference from HFD-Vehicle. ⁺, Denotes statistically significant difference from LFD-Vehicle mice. N = 7–14 mice per group.</p

MnTBAP treatment increase insulin-stimulated PKB phosphorylation and content in muscles from mice fed a HFD.

<p>Mice were fed a LFD or HFD for 5 months and then treated with or without MnTBAP (10 mg/kg) daily for 5 weeks. At 15 min following an intraperitoneal injection of insulin (2 U/Kg), quadriceps muscles were excised and rapidly frozen in liquid nitrogen for subsequent Western blot analyses. <b>(A)</b> Representative PKB immunoblots for pThr³⁰⁸ and pSer⁴⁷³ PKB as well as total PKB-α and PKB-β isoforms. GAPDH was used as a loading control. Quantification of <b>(B)</b> pThr³⁰⁸ PKB, <b>(C)</b> pSer³⁰⁸ PKB, <b>(D)</b> PKB-α and <b>(E)</b> PKB-β content, each normalized to GAPDH. *, Denotes statistically significant difference from HFD-Vehicle. N = 5–6 mice per group.</p

SnMP improves MnTBAP’s ability to reduce blood glucose levels during IAGT tests.

<p>Mice were fed a LFD or HFD for 5 months and then treated with MnTBAP (10 mg/kg) alone or in combination with SnMP (20 mg/Kg) daily for 5 weeks. Mice were simultaneously injected with 2.0 U/Kg insulin and 2.0 g/Kg glucose and blood glucose values were assessed at baseline and 20, 40, and 60 min following the injection (Panels A) and the area under the IAGT curve was calculated (Panels B). *, Denotes statistically significant difference from HFD-Vehicle. ⁺, Denotes statistically significant difference from LFD-Vehicle mice. ^#, Denotes statistically significant difference from HFD-MnTBAP mice. N = 5 mice per group.</p

MnTBAP treatment alters insulin-stimulated PKB phosphorylation but not content in EWAT from mice fed a HFD.

<p>Mice were fed a LFD or HFD for 5 months and then treated with or without MnTBAP (10 mg/kg) daily for 5 weeks. At 15 min following an intraperitoneal injection of insulin (2 U/Kg), EWAT was excised and rapidly frozen in liquid nitrogen for subsequent Western blot experiments. <b>(A)</b> Representative PKB immunoblots for pThr³⁰⁸ and pSer⁴⁷³ PKB as well as total PKB-α and PKB-β isoforms. α-tubulin was used as a loading control. Quantification of <b>(B)</b> pThr³⁰⁸ PKB, <b>(C)</b> pSer³⁰⁸ PKB, <b>(D)</b> PKB-α and <b>(E)</b> PKB-β content, each normalized to α-tubulin. *, Denotes statistically significant difference from HFD-Vehicle. ⁺, Denotes statistically significant difference from LFD-MnTBAP. N = 5–6 mice per group.</p

MnTBAP treatment reduces body weight and adipose tissue mass in mice fed a LFD or HFD.

<p>Mice were fed a LFD or HFD for 5 months and then treated with or without MnTBAP (10 mg/kg) daily for 5 weeks. <b>(A)</b> Fed-state body weights at different treatment durations. <b>(B)</b> Fasted body weights before and after MnTBAP or vehicle treatments. <b>(C)</b> Epididymal white adipose tissue (EWAT) mass. *, Denotes statistically significant difference from respective vehicle-treated mice. ⁺, Denotes statistically significant difference from respective LFD mice. N = 5–6 mice per group.</p

The effects of a HFD and MnTBAP treatment on energy expenditure, oxygen consumption, and RER in mice.

<p>Indirect calorimetry using open circuit spirometry was conducted for 24 hours 3 weeks into the MnTBAP treatment course for the assessment of energy expenditure <b>(A),</b> oxygen consumption <b>(B),</b> and RER values <b>(C)</b>. *, Denotes statistically significant difference from the all other groups respective of light/dark cycle. N = 6 mice per group.</p