Additional file 1: Tables S1 and S2. of Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects

Pearson correlation coefficients matrix for inflammatory biomarkers’ expression in the lung submucosa after single or co-exposure to diesel exhaust and allergen. (PDF 213 kb

Additional file 2: Figure S1. of Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects

Immunohistochemical staining of positive and negative controls. A) Representative 40X image of positive staining using mAb AA1 for tryptase in human tonsil tissue; B) Representative 40X image of positive staining using mAb EG2 for ECP in human tonsil tissue; C) Representative 40X image of positive staining using mAb NP57 for neutrophil elastase in human tonsil tissue; D) Representative 40X image of positive staining using mAb B-A38 for CD138 in human lung tissue; E) Representative 40X image of positive staining using mAb 4B12 for CD4 in human tonsil tissue; F) Representative 40X image of positive staining using mAb 4D9 for IL-4 in human tonsil tissue; G) Representative 40X image of isotype control using mAb mouse IgG1 (0.07 μg.mL−1) in human lung tissue; H) Representative 40X image of isotype control staining using mAb mouse IgG1 (20.0 μg.mL−1) in human lung tissue. (PDF 482 kb

Additional file 1: of Gene expression analysis in asthma using a targeted multiplex array

Supplementary Methods – Methods describing selection of house keeping genes and immunohistochemical staining procedure. Supplementary Tables – Tables containing clinical demographics for subjects, average counts, fold change, and p-value for all genes studied, and all differentially co-expressed genes. Supplementary Figures and Legends – Figures showing sample immunohistochemical staining for proteins of significantly altered genes, co-expression plots. (DOCX 35 kb