Length dependence of Chd1 effects on H3 replacement.

<p>H3 replacement was averaged for the 500 bp at the 5′ ends of genes (A), or the 3′ ends of genes (B). Genes were ordered by length, and an 80 gene window average is shown for wild type and <i>chd1Δ</i> turnover data as indicated. Bottom panel plots gene lengths, and locations for 1, 2, and 3 kb are indicated below panel (B).</p

Chd1 affects H3.3_core-GFP localization on Chd1 in <i>Drosophila</i>.

<p>(A) Representative sections from confocal imaging of H3.3_core-GFP in nuclei from salivary glands of wild type larvae, (B) <i>chd1⁵</i> heterozygotes and (C) <i>chd1⁵</i> homozygotes. The GFP signal is pseudo green. In all cases, H3.3_core-GFP was expressed from <i>P[UHS-H3.3_core-GFP]</i> and driven by <i>P{GawB} AB1-Gal4</i>. (D) Quantitation of banding patterns observed in nuclei from flies with the indicated genotypes. A total of 44 wild type, 144 heterozygote, and 162 homozygous null nuclei were scored, all blind to genotype.</p

The H3 N-terminal tail functions redundantly with Chd1 and an H3.3-like surface of histone H3 in budding yeast.

<p>The indicated histone H3 plasmids (which also carried histone H4) were transformed into wild type <i>CHD1</i> or <i>chd1</i> null strains that lack both chromosomal copies of the histone H3/H4 genes and contained a <i>URA3 H3/H4</i> plasmid. Cultures were adjusted to 1×10⁷ cells per ml and five-fold serial dilutions were spotted directly onto 5FOA media, selecting for cells that had lost the <i>URA3 H3/H4</i> plasmid, and incubated for 2 days at 30°C.</p

Chd1 effects on H3 methylation patterns.

<p>H3K4me3 and H3K36me3 were mapped genome-wide by ChIP-chip on tiling microarrays. Metagene analysis is shown for wild type and <i>chd1Δ</i> strains, as indicated.</p