DojoIBL: Online Inquiry-based Learning

DojoIBL is a web based platform to support collaborative inquirybased learning processes. It imitates real-world research processes and organizes inquiry activities into several phases. DojoIBL considers lessons learned from the weSPOT project and offers a cloud-based highly scalable infrastructure that has a strong focus on (mobile) data collection. In this sense, DojoIBL blends formal (desk-top based) learning and informal (mobile) learning. Within the course of 1 year, a design based research methodology was implemented in 10 national and international inquiry projects. Within this period, students were inter-viewed at regular times. Time and task management issues turned out to be critical functionalities and were thus implemented in several iterations

Bloom of a denitrifying methanotroph, 'Candidatus Methylomirabilis limnetica', in a deep stratified lake

Methanotrophic bacteria represent an important biological filter regulating methane emissions into the atmosphere. Planktonic methanotrophic communities in freshwater lakes are typically dominated by aerobic gamma-proteobacteria, with a contribution from alpha-proteobacterial methanotrophs and the NC10 bacteria. The NC10 clade encompasses methanotrophs related to 'Candidatus Methylomirabilis oxyfera', which oxidize methane using a unique pathway of denitrification that tentatively produces N-2 and O-2 from nitric oxide (NO). Here, we describe a new species of the NC10 clade, 'Ca. Methylomirabilis limnetica', which dominated the planktonic microbial community in the anoxic depths of the deep stratified Lake Zug in two consecutive years, comprising up to 27% of the total bacterial population. Gene transcripts assigned to 'Ca. M. limnetica' constituted up to one third of all metatranscriptomic sequences in situ. The reconstructed genome encoded a complete pathway for methane oxidation, and an incomplete denitrification pathway, including two putative nitric oxide dismutase genes. The genome of 'Ca. M. limnetica' exhibited features possibly related to genome streamlining (i.e. less redundancy of key metabolic genes) and adaptation to its planktonic habitat (i.e. gas vesicle genes). We speculate that 'Ca. M. limnetica' temporarily bloomed in the lake during non-steady-state conditions suggesting a niche for NC10 bacteria in the lacustrine methane and nitrogen cycle