Effects of Stabilizers and Water Chemistry on Arsenate Sorption by Polysaccharide-Stabilized Magnetite Nanoparticles

Magnetite nanoparticles were synthesized with starch and sodium carboxymethyl cellulose (CMC) as a stabilizer, and tested for enhanced arsenate removal. Starch at ≥0.04 wt % or CMC at ≥0.005 wt % stabilized 0.1 g/L (as Fe) of the nanoparticles. While CMC-stabilized magnetite displays a highly negative zeta (ζ) potential, starch-stabilized magnetite shows a nearly neutral surface. Increasing the starch concentration from 0 to 0.04 wt % doubles the arsenate uptake, yet the nanoparticles remain settleable by gravity. Further increasing the starch concentration to 0.1 wt % results in fully dispersed nanoparticles and increases the arsenate uptake by 14%. Starch-stabilized magnetite offers a much faster sorption rate and greater capacity than CMC-stabilized magnetite. The sorption kinetics can be modeled using an intraparticle-diffusion model. The sorption capacity increases with decreasing pH. Dissolved organic matter at 20 mg/L as TOC decreases the arsenate uptake by 19.1%. When aged for >1.5 years, the nanoparticles did not show any arsenate leaching or particle dissolution

Comparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in the Eyes of High Myopic Patients

Myopia accounts for a significant proportion of visual lesions worldwide and has the potential to progress toward pathological myopia. This study aims to reveal the difference in protein content in aqueous humor between high myopic and nonhigh myopic patients, as well as better understand the dysregulation of proteins in myopic eyes. Aqueous humor was collected for liquid chromatograph mass spectrometer (LC/MS) analysis from 30 individual eyes that underwent phacoemulsification and intraocular lens (IOL) implantation. Results showed that a total of 190 differentially expressed proteins were identified, which revealed their involvement in cell metabolism, immune and inflammatory response, and system and anatomical structure. Further analysis focused on 15 intensively interacted hub proteins, encompassing functions related to complement cascades, lipoprotein metabolism, and fibrin biological function. Subsequent validations demonstrated elevated levels of APOE (apolipoprotein E), C3 (complement 3), and AHSG (α-2-HS-glycoprotein) in the high myopia group (31 eyes of cataracts and 45 eyes of high myopia with cataracts). AHSG had a significant positive correlation with axial length in high myopic patients, with good efficacy in distinguishing between myopic and nonmyopic groups. AHSG may be a potential indicator of the pathological severity and participator in the pathological progress of high myopia. This study depicted differential expression characteristics of aqueous humor in patients with high myopia and provided optional information for further experimental research on exploring the molecular mechanisms and potential therapeutic targets for high myopia. Data are available via ProteomeXchange with the identifier PXD047584