Protective effects of fasudil hydrochloride post-conditioning on acute myocardial ischemia/reperfusion injury in rats

Background: In recent years, the alleviation of acute myocardial ischemia and reperfusioninjury (MI/RI) during myocardial reperfusion has presented a significant clinical challenge.This study was performed to investigate the effects of fasudil hydrochloride (FH) postconditioningon MI/RI and the underlying mechanism.Methods: Seventy-two rats were randomly divided into four groups: a Sham group, anischemia/reperfusion (I/R) group, a fasudil hydrochloride (FH) group, and a fasudilhydrochloride+PI3K inhibitor (FH+I) group. Myocardial infarct size, cell apoptotic index(AI), and myocardial tissue expression of Rho-associated coiled-coil containing proteinkinase 1 (ROCK1), Bcl-2, Bcl-2 associated X protein (Bax), caspase-3, Akt and phosphorylatedAkt (P-Akt) were detected.Results: All these parameters, except Akt expression, were higher in the I/R group than in theSham group (p < 0.05). Compared to the I/R group, myocardial infarct size, AI, Bax andcaspase-3 expression were significantly reduced in the FH group (p < 0.05), while Bcl-2expression was increased (p < 0.05). However, the myocardial infarct size and AI of the FH+Igroup were similar to those of the I/R group (p > 0.05). Compared to the FH group, Bcl-2expression was reduced in the FH+I group, while Bax and caspase-3 expression was increased(p < 0.05). Furthermore, P-Akt expression in the FH group was significantly higherthan that of the I/R group (p < 0.05).Conclusions: FH post-conditioning alleviated MI/RI, with narrowing of the infarct size anddecreased apoptosis of ischemic cardiocytes. The mechanism was associated with activation ofthe PI3K-Akt signaling pathway

Poly[[aqua­bis(μ3-isonicotinato-κ3 O:O′:N)tris­(μ2-isonicotinato-κ3 O,O′:N)(nitrato-κO)bis­(μ4-oxalato-κ6 O 1,O 2:O 2:O 1′,O 2′:O 1′)dierbium(III)tetra­silver(I)] tetra­hydrate]

In the title coordination polymer, {[Ag4Er2(C6H4NO2)5(C2O4)2(NO3)(H2O)]·4H2O}n, each ErIII atom is coordinated in a bicapped trigonal–prismatic coordination geometry by three O atoms from two isonicotinate (IN) ligands, four O atoms from two oxalate ligands and one O atom from either a nitrate ion or a water mol­ecule, both of which are half-occupied over the same site. One AgI atom has a Y-shaped geometry defined by one N atom from one IN ligand, one O atom from another IN ligand and one O atom from an oxalate ligand. The other AgI atom is coordinated by two IN ligands and one O atom from an oxalate ligand. One of the IN ligands is disordered over an inversion center and forms a bridge between two centrosymmetric AgI ions. Due to the disorder, this IN ligand coordinates to the Ag atom through either the pyridyl N or the carboxyl­ate O atoms. The IN and oxalate ligands link the Er and Ag atoms into a three-dimensional coordination framework. O—H⋯O and C—H⋯O hydrogen bonds are observed in the crystal structure

Effect of insulin pump infusion on comprehensive stress state of patients with diabetic ketoacidosis

Purpose: To assess changes in the comprehensive stress state of patients with diabetic ketoacidosis (DKA) following treatment with insulin infusion pump.Methods: A total of 240 patients suffering from DKA who received treatment at The First Affiliated Hospital of Zhengzhou University from Feb. 2011 to Feb. 2014 were selected randomly and divided into a control group (120 cases), administered continuous intravenous insulin, and a treatment group (120 cases), administered insulin with an infusion pump. Relevant diabetes-associated serum indices, oxidative stress and stress hormone levels were compared between the groups at different time points.Results: Levels of glycosylated haemoglobin (HbAlc), fasting blood glucose (FBG) and anion gap (AG) were lower (p < 0.05) in the treatment group than in the control group at various time points. Carbon dioxide combining power (CO2-CP) and C-reactive peptide levels in the treatment group were higher (p < 0.05) than those of the control group at different time points. Except for the level of methane dicarboxylic aldehyde (MDA), the values of other oxidative stress indices were higher (p < 0.05) in the treatment group than in the control group at various time points. Moreover, the treatment group had lower serum cortisol (Cor) and noradrenalin (NA) levels and higher levels of free triiodothyronine (FT3), free thyroxine (FT4) and thyroid-stimulating hormone (TSH) than those of (p < 0.05).Conclusion: Insulin pump infusion effectively relieves the comprehensive stress state of patients suffering from DKA, and its therapeutic effect is superior to that of continuous instillation of low-dose insulin.Keywords: Diabetic ketoacidosis, Insulin pump, Comprehensive stress state, Blood glucose, Carbon dioxide combining power, C-reactive peptid

1-[1-(Hydroxy­imino)eth­yl]-N-(2-methoxy­phen­yl)cyclo­propane­carboxamide

The title compound, C13H16N2O3, adopts an E configuration with respect to the C=N bond and an intra­molecular N—H⋯N hydrogen bond results in the formation of a six-membered ring. In the crystal, inter­molecular O—H⋯O hydrogen bonds link the mol­ecules into a chain propagating along the b axis. Very weak π–π stacking inter­actions [centroid–centroid distance = 4.18 (2) Å] may further consolidate the packing, forming a two-dimensional supra­molecular network