Evaluation of bread crumbs as a potential carbon source for the growth of Thraustochytrid species for oil and omega-3 production

The utilization of food waste by microorganisms to produce omega-3 fatty acids or biofuel is a potentially low cost method with positive environmental benefits. In the present study, the marine microorganisms Thraustochytrium sp. AH-2 and Schizochytrium sp. SR21 were used to evaluate the potential of breadcrumbs as an alternate carbon source for the production of lipids under static fermentation conditions. For the Thraustochytrium sp. AH-2, submerged liquid fermentation with 3% glucose produced 4.3 g/L of biomass and 44.16 mg/g of saturated fatty acids after seven days. Static fermentation with 0.5% and 1% breadcrumbs resulted in 2.5 and 4.7 g/L of biomass, and 42.4 and 33.6 mg/g of saturated fatty acids, respectively. Scanning electron microscopic (SEM) studies confirmed the growth of both strains on breadcrumbs. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy for both strains were consistent with the utilization of breadcrumbs for the production of unsaturated lipids, albeit at relatively low levels. The total lipid yield for static fermentation with bread crumbs was marginally lower than that of fermentation with glucose media, while the yield of unsaturated fatty acids was considerably lower, indicating that static fermentation may be more appropriate for the production of biodiesel than for the production of omega-3 rich oils in these strains

Cache County Water Demand/Supply Model

This report descibes a municipal water demand forecasting model for use in areas of mixed rural and urban housing types. A series of residential demand functions were derived which forecast water demand based on the ype and density of housing and season. Micro sampling techniques were used to correlate water use data and explanatory variable data for low, medium, and high density housing. The demand functions were incorporated into a geographic information system (GIS) platform cosisting of a desk-top mapping program, MapInfo, coupled with a user interface program written in Visual Basic. The GIS-based model analyzes water demand at the census block level and aggregates the block level demands to a total city residential water demand. Averaged values of explanatory variables for each block are derived using the spacial relations of the block to map objects which have as attributes the various explanatory variable data. The model was applied to each of 23 community water systems in Cache County, Utah. The model projects future demands to the year 2020 based upon the individual community growth rate estimates produced by the Utah state demographers. In addition to projecting future demands, the model includes a supply allocation module which matches each systme\u27s demand with individual water supply sources

University small satellite program-ANUSAT

Indian Space Research Organization (ISRO) over the last 3 decades has steadily progressed towards establishment of space system for communication, Earth observation and disaster warning for national development. Also, the launchers for placing both Earth observation and communication satellites have been developed. While these efforts are being sustained on a continuous basis, ISRO initiated Small Satellite Program few years ago for demonstration of new technologies that can be adopted to operational mission as well as Small Satellite Earth Observation program to complement the existing earth observation mission. Also realizing the importance of steady flow of engineers and scientists in Space Technology, ISRO has been pursuing to promote development of micro-satellite at Indian universities that will provide hands-on experience to the university students in Space Technology and also developing new technologies for the future. Anna University at Chennai, India, has taken up the first micro-satellite program within the university environment. The micro-satellite development will be carried out at Aerospace Research Centre in Anna University with review and funding support from ISRO. The project will be implemented through involvement of students, researchers and faculty staff. The satellite is a simple spinning satellite carrying store and forward payload being the first mission and weighs less than 50 kg. The Project has commenced in January 2002. The preliminary design review of the satellite has been completed in December 2002. The micro-satellite is scheduled for launch in 2005 as an auxiliary payload in ISRO's launcher PSLV (Polar Satellite Launch Vehicle)

Patronin-mediated minus end growth is required for dendritic microtubule polarity.

Microtubule minus ends are thought to be stable in cells. Surprisingly, in Drosophila and zebrafish neurons, we observed persistent minus end growth, with runs lasting over 10 min. In Drosophila, extended minus end growth depended on Patronin, and Patronin reduction disrupted dendritic minus-end-out polarity. In fly dendrites, microtubule nucleation sites localize at dendrite branch points. Therefore, we hypothesized minus end growth might be particularly important beyond branch points. Distal dendrites have mixed polarity, and reduction of Patronin lowered the number of minus-end-out microtubules. More strikingly, extra Patronin made terminal dendrites almost completely minus-end-out, indicating low Patronin normally limits minus-end-out microtubules. To determine whether minus end growth populated new dendrites with microtubules, we analyzed dendrite development and regeneration. Minus ends extended into growing dendrites in the presence of Patronin. In sum, our data suggest that Patronin facilitates sustained microtubule minus end growth, which is critical for populating dendrites with minus-end-out microtubules

Work and Time analysis for rendering terms in a thesaurus derived from a faceted classification schedule : a case study with Lathe Production

Describes the various steps involved in rendering a standard term. Identifies the difference in the role of the term occuring in a faceted classification schedule. Analyses the work involved in the conversion, and the time required for each of the steps

Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca(2+) uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca(2+) influx and localizes to membranes that are destined to undergo fusion during mating

Generation of polarization entangled photons using type-II doubly periodically poled lithium niobate waveguides

In this paper, we address the issue of the generation of non-degenerate cross-polarization-entangled photon pairs using type-II periodically poled lithium niobate. We show that, by an appropriate engineering of the quasi-phase-matching grating, it is possible to simultaneously satisfy the conditions for two spontaneous parametric down-conversion processes, namely ordinary pump photon down-conversion to either extraordinary signal and ordinary idler paired photons, or to ordinary signal and extraordinary idler paired photons. In contrast to single type-II phase-matching, these two processes, when enabled together, can lead to the direct production of cross-polarization-entangled state for non degenerate signal and idler wavelengths. Such a scheme should be of great interest in applications requiring polarization-entangled non degenerate paired photons with, for instance, one of the entangled photons at an appropriate wavelength being used for local operation or for quantum storage in an atomic ensemble, and the other one at the typical wavelength of 1550 nm for propagation through an optical fiber.Comment: 9 pages, 5 figures, 2 table

Ultrasensitive Optical Shape Characterization of Gold Nanoantennas Using Second Harmonic Generation

Second harmonic generation from plasmonic nanoantennas is investigated numerically using a surface integral formulation for the calculation of both the fundamental and the second harmonic electric field. The comparison between a realistic and an idealized gold nanoantenna shows that second harmonic generation is extremely sensitive to asymmetry in the nanostructure shape even in cases where the linear response is barely modified. Interestingly, minute geometry asymmetry and surface roughness are clearly revealed by far-field analysis, demonstrating that second harmonic generation is a promising tool for the sensitive optical characterization of plasmonic nanostructures. Furthermore, defects located where the linear field is strong (e.g., in the antenna gap) do not necessarily have the strongest impact on the second harmonic signal