2 research outputs found
CONGENITAL REDUCTION IN NUMBER OF NEPHRONS IMPAIRS PROXIMAL TUBULAR GROWTH IN THE CONTRALATERAL KIDNEY DURING PARTIAL UNILATERAL URETERAL OBSTRUCTION
PURPOSE
To compare the response of the contralateral (CL) kidney during partial unilateral uretaral obstruction (PUO) and from obstruction/release (PUO/Rel) to sham-operated kidneys in both wild-type (WT) mice and mice with 50% reduction of nephron number (Os/+)
MATERIAL AND METHODS
C57/BL6 WT and mice with oligosyndactylism and 50% reduction of nephron number (Os/+), were subjected to sham operation or PUO in the first 2 days of life. Additional mice underwent release of obstruction at 7 days (PUO-Rel). All kidneys were harvested at 6 weeks (adulthood). Using histomorphometry, glomerular number and area were determined by PAS staining, and proximal tubular (PT) mass and injury to the glomerulotubular junction (GTJ) were measured in Lotus tetragonolobus lectin-stained kidneys. Interstitial collagen deposition was quantitated using Picrosirius red staining
RESULTS
kidney weight was not different between groups. Compared to WT, glomerular number was lower in all groups of Os/+ mice, and glomerular area increased in Sham and contralateral kidney following release (CL/Rel) Os/+ kidneys, but not in CL Os/+ kidneys with persistent PUO. Compared to Sham, PT mass decreased in CL Os/+ kidney, but not in CL/Rel kidney. The % of intact GTJ in CL kidneys was not different between groups. Deposition of interstitial collagen was increased in CL kidney regardless of strain.
CONCLUSIONS
compared to Sham, CL and CL/Rel kidney showed no difference in kidney weight, glomerular number, glomerular area, and % of intact GTJ in both strains. The 50% reduction in the number of nephrons reduced fractional PT mass in CL kidney, which was prevented by release of PUO. Obstruction increased interstitial collagen in both strains. We conclude that reduction in the number of nephrons impairs adaptation to contralateral PUO, and that early release of obstruction preserves CL kidney PT mass. Thus, timely release of PUO may be of particular importance in the context of prematurity or contralateral renal hypoplasia
Diagnosis and treatment of prostatic abscess
OBJECTIVES: Present and discuss the pathogenesis, diagnostic methods and treatment of the prostatic abscess. MATERIALS AND METHODS: We have retrospectively studied the medical records of 9 patients diagnosed and treated for prostatic abscess, between March 1998 and December 2000, assessing age, context, associated diseases, and diagnostic and therapeutic methods. We have compared the data found with those described in literature, based on Medline data. RESULTS: Mean age was 52.6 years. Three patients had previous diabetes mellitus diagnosis, and one was infected by HIV virus. Transrectal ultrasound of the prostate confirmed the diagnosis of prostatic abscess in all 7 cases in which it was performed. All cases received antibiotic treatment, and 77.8% needed concomitant surgical treatment. Two cases of microabscess were treated only with antibiotics. Four patients were submitted to perineal catheter drainage, 2 were submitted to transurethral resection of the prostate (TURP), and one patient required both procedures. Mean hospitalization time was 11.2 days, and most frequent bacterial agent was S. aureus. All patients were discharged from the hospital, and there was no death in this series. CONCLUSIONS: Prostatic abscess should be treated with broad-spectrum antibiotics and surgical drainage (perineal puncture or TURP). Microabscess may heal without surgery