Adult nutrition and butterfly fitness: effects of diet quality on reproductive output, egg composition, and egg hatching success

<p>Abstract</p> <p>Background</p> <p>In the Lepidoptera it was historically believed that adult butterflies rely primarily on larval-derived nutrients for reproduction and somatic maintenance. However, recent studies highlight the complex interactions between storage reserves and adult income, and that the latter may contribute significantly to reproduction. Effects of adult diet were commonly assessed by determining the number and/or size of the eggs produced, whilst its consequences for egg composition and offspring viability were largely neglected (as is generally true for insects). We here specifically focus on these latter issues by using the fruit-feeding tropical butterfly <it>Bicyclus anynana</it>, which is highly dependent on adult-derived carbohydrates for reproduction.</p> <p>Results</p> <p>Adult diet of female <it>B. anynana </it>had pronounced effects on fecundity, egg composition and egg hatching success, with butterflies feeding on the complex nutrition of banana fruit performing best. Adding vitamins and minerals to a sucrose-based diet increased fecundity, but not offspring viability. All other groups (plain sucrose solution, sucrose solution enriched with lipids or yeast) had a substantially lower fecundity and egg hatching success compared to the banana group. Differences were particularly pronounced later in life, presumably indicating the depletion of essential nutrients in sucrose-fed females. Effects of adult diet on egg composition were not straightforward, indicating complex interactions among specific compounds. There was some evidence that total egg energy and water content were related to hatching success, while egg protein, lipid, glycogen and free carbohydrate content did not seem to limit successful development.</p> <p>Conclusion</p> <p>The patterns shown here exemplify the complexity of reproductive resource allocation in <it>B. anynana</it>, and the need to consider egg composition and offspring viability when trying to estimate the effects of adult nutrition on fitness in this butterfly and other insects.</p

Environmental Effects on Temperature Stress Resistance in the Tropical Butterfly Bicyclus Anynana

BACKGROUND: The ability to withstand thermal stress is considered to be of crucial importance for individual fitness and species' survival. Thus, organisms need to employ effective mechanisms to ensure survival under stressful thermal conditions, among which phenotypic plasticity is considered a particularly quick and effective one. METHODOLOGY/PRINCIPAL FINDINGS: In a series of experiments we here investigate phenotypic adjustment in temperature stress resistance following environmental manipulations in the butterfly Bicyclus anynana. Cooler compared to warmer acclimation temperatures generally increased cold but decreased heat stress resistance and vice versa. In contrast, short-time hardening responses revealed more complex patterns, with, e.g., cold stress resistance being highest at intermediate hardening temperatures. Adult food stress had a negative effect on heat but not on cold stress resistance. Additionally, larval feeding treatment showed interactive effects with adult feeding for heat but not for cold stress resistance, indicating that nitrogenous larval resources may set an upper limit to performance under heat stress. In contrast to expectations, cold resistance slightly increased during the first eight days of adult life. Light cycle had marginal effects on temperature stress resistance only, with cold resistance tending to be higher during daytime and thus active periods. CONCLUSIONS/SIGNIFICANCE: Our results highlight that temperature-induced plasticity provides an effective tool to quickly and strongly modulate temperature stress resistance, and that such responses are readily reversible. However, resistance traits are not only affected by ambient temperature, but also by, e.g., food availability and age, making their measurement challenging. The latter effects are largely underexplored and deserve more future attention. Owing to their magnitude, plastic responses in thermal tolerance should be incorporated into models trying to forecast effects of global change on extant biodiversity

Initial psychometric validation of the questionnaire on pain caused by spasticity (QPS)

Abstract Background The Questionnaire on Pain caused by Spasticity (QPS) is a modular patient- and observer-reported outcome measure of spasticity-related pain (SRP) in children with cerebral palsy (CP). Originally developed for an English-speaking population, we conducted a psychometric validation of a recently developed Chinese language version of the QPS. Methods This was a prospective, observational study involving 137 children/adolescents with CP and upper and/or lower limb spasticity and their parents at three sites in China. Six QPS modules were used, three each for upper and lower limb SRP assessment: a patient self-report module; an interviewer-administered module used by site staff based on the cognitive, communicative, and motor abilities of a patient; and a parent/caregiver module administered for all children as an observer-reported outcome to complement the patient-reported outcome. If no assessment by the patient was possible because of age or cognitive impairments, only the parent/caregiver module was completed. Two visits with a 3-week interval provided data to evaluate and establish administrative ease of use, scoring of the QPS (factor analyses, Rasch analyses), reliability (Cronbach’s α, intraclass correlation coefficient), validity (correlations with quality of life [PedsQL™], motor impairment [Gross Motor Function Classification System, Gross Motor Function Measure-66, Manual Ability Classification System], and spasticity [Ashworth Scale, Modified Tardieu Scale]). Results For most children, clinic staff reported no difficulties associated with general QPS use or deciding which module to use. Children (and parents) who reported more demanding activities also reported higher levels of associated SRP (or observed SRP behavior). Activity-related SRP items were combined for a total QPS score. Cronbach’s α was low for child self-report, but was acceptable for interviewer-administered and parent reports on SRP. Test–retest reliability was high for all modules. Moderate–strong associations were frequently seen between QPS and quality of life, and were particularly strong in the child self-report group. Relatively weak associations were observed between QPS and motor impairment and spasticity. Conclusions This first study was successful in providing initial evidence for the psychometric properties. Clinic staff were able to administer the QPS modules easily, and both children and parents were able to complete the designated QPS appropriately

Adult nutrition and butterfly fitness: effects of diet quality on reproductive output, egg composition, and egg hatching success-2

Early: Eggs from days 3–4 of adult life; late: eggs from days 16–17. For explanations of dietary treatments see Fig. 1.<p><b>Copyright information:</b></p><p>Taken from "Adult nutrition and butterfly fitness: effects of diet quality on reproductive output, egg composition, and egg hatching success"</p><p>http://www.frontiersinzoology.com/content/5/1/10</p><p>Frontiers in Zoology 2008;5():10-10.</p><p>Published online 10 Jul 2008</p><p>PMCID:PMC2481257.</p><p></p

Adult nutrition and butterfly fitness: effects of diet quality on reproductive output, egg composition, and egg hatching success-1

to adult feeding and female age. Early: Eggs from days 3–4 of adult life; late: eggs from days 16–17. For explanations of dietary treatments see Fig. 1.<p><b>Copyright information:</b></p><p>Taken from "Adult nutrition and butterfly fitness: effects of diet quality on reproductive output, egg composition, and egg hatching success"</p><p>http://www.frontiersinzoology.com/content/5/1/10</p><p>Frontiers in Zoology 2008;5():10-10.</p><p>Published online 10 Jul 2008</p><p>PMCID:PMC2481257.</p><p></p