СПЕКТРОСКОПІЯ ЯДЕРНОГО МАГНІТНОГО РЕЗОНАНСУ В АНАЛІЗІ ОБ'ЄКТІВ ДОВКІЛЛЯ

Additional file 3. Multiplication rate

MOESM1 of Cellulose filtration of blood from malaria patients for improving ex vivo growth of Plasmodium falciparum parasites

Additional file 1. Patient characteristics from the children’s ward at Korogwe District Hospital

Mean anti-gSG6 IgG level per subvillage, plotted against increasing mosquito exposure per subvillage.

<p>Anti-gSG6 IgG levels are given as the log-10 adjusted mean anti-gSG6 OD per subvillage. Mosquito exposure is given as the ascending and sequential mean <i>Anopheles</i> female count for each of 15 subvillages (x-axis), as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040170#pone-0040170-g002" target="_blank"><i>Figure 2</i></a>. The trend-line from the linear regression is shown as a dashed line (r² = 0.436, p = 0.007).</p

IgG responses to gSG6 and <i>P. falciparum</i> antigens, plotted against malaria incidence after serum collection.

<p>Malaria incidence is grouped into 0 episodes, 1–2 episodes or >3 episodes. <b>A.</b> Box plots showing anti-gSG6 IgG level between groups sorted according to malaria incidence subsequent to serological sampling. Boxes, whiskers and P values are as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040170#pone-0040170-g004" target="_blank"><i>Figure 4</i></a>. n = 269. <b>B.</b> Seroprevalence of anti-gSG6 and anti-<i>P. falciparum</i> IgG antibodies plotted against grouped malaria incidence. Sample sizes vary by antigen according to the available serological methodology; CSP n = 246, AMA-1 n = 227, MSP-1 n = 227, MSP-3 n = 566, GLURP R1 n = 566, GLURP R2 n = 566. P values were determined by a test for trend in proportions (***). Error bars denote 95% CI.</p

Seroprevalence and IgG antibody levels among seropositive children to <i>An. gambiae</i> gSG6, and <i>P. falciparum</i> CSP, AMA-1, MSP-1, MSP-3, GLURP R1 and GLURP R2.

<p><b>OD</b> optical density.</p><p><b>IQR</b> inter-quartile range (25^th and 75^th percentiles).</p><p><b>n/N</b> proportion of seropositive individuals/total sample size.</p>*<p>seropositive individuals only.</p

Mean household <i>Anopheles</i> female count during peak transmission (May) in different subvillages.

<p>Numbers of households sampled for each subvillage, in order of <i>Anopheles</i> exposure, were as follows: MY = 45, MS = 23, MA = 26, MU = 21, MJ = 29, KS = 24, LW = 65, LU = 61, MD = 45, MK = 14, KW = 99, SH = 13, GE = 47, KI = 30, MG = 91.</p

IgG responses to gSG6 and <i>P. falciparum</i> antigens, grouped into quintiles of household <i>Anopheles</i> exposure.

<p><b>A.</b> Box plots showing anti-gSG6 IgG level between groups sorted according to <i>Anopheles</i> exposure in quintiles. Boxes show the median and 25^th/75^th percentiles, whiskers show the 5^th/95^th percentiles, and outliers are represented by dots. Where outliers were excluded from the graph but not analysis they are marked with a + and included in parentheses. P values for pairwise comparisons were determined by Mann-Whitney test with Bonferroni correction (*), and for all groups by Kruskal-Wallis test (**). <b>B.</b> Seroprevalence of anti-gSG6 and anti-<i>P. falciparum</i> IgG antibodies plotted against <i>Anopheles</i> exposure in quintiles. Error bars indicate 95% confidence intervals (CI). P values were determined by a test for trend in proportions (***).</p