6,323 research outputs found

    Microbial genomic taxonomy

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    A need for a genomic species definition is emerging from several independent studies worldwide. In this commentary paper, we discuss recent studies on the genomic taxonomy of diverse microbial groups and a unified species definition based on genomics. Accordingly, strains from the same microbial species share >95% Average Amino Acid Identity (AAI) and Average Nucleotide Identity (ANI), >95% identity based on multiple alignment genes, 70% in silico Genome-to-Genome Hybridization similarity (GGDH). Species of the same genus will form monophyletic groups on the basis of 16S rRNA gene sequences, Multilocus Sequence Analysis (MLSA) and supertree analysis. In addition to the established requirements for species descriptions, we propose that new taxa descriptions should also include at least a draft genome sequence of the type strain in order to obtain a clear outlook on the genomic landscape of the novel microbe. The application of the new genomic species definition put forward here will allow researchers to use genome sequences to define simultaneously coherent phenotypic and genomic groups

    Amniotic-Fluid Ingestion Enhances the Central\ud Analgesic Effect of Morphine

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    Amniotic fluid and placenta contain a substance (POEF) that when ingested enhances opioid-mediated analgesia produced by several agents (morphine injection, vaginal/cervical stimulation, late pregnancy, footshock), but not that produced by aspirin injection. The present series of experiments employed quaternary naltrexone, an opioid antagonist that does not readily cross the blood-brain barrier, in conjunction with either peripheral or central administration of morphine, to determine whether amniotic-fluid ingestion (and therefore POEF ingestion) enhances opioid-mediated analgesia by affecting the central and/or peripheral actions of morphine. The results suggest that POEF affects only the central analgesic effects of morphine

    A history of the Laura Ann Sisk Memorial Reading Clinic

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    It was the purpose of this study (1) to present to the College of the Pacific a written record of the history of the Laura Ann Sisk Memorial Reading Clinic since its inception; (2) to trace its growth in scope and philosophy by portraying past and present methods and techniques employed; (3) to analyze the results of clinic practices which were obtained systematically; and (4) to discuss the future plans for the development of the clini

    Corss-Age Tutoring Program

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    This master\u27s project provided a teachers~ handbook to assist in establishing a cross-age tutoring program. In addition to a philosophy statement and a review of the literature, this project also .provided forms for implementing the program and materials for training the tutors. This manual was prepared for use in an elementary school setting and contains a step-by-step approach to establish a cross-age tutoring program

    The lived experience of non-offending mothers in cases of intrafamilial child sexual abuse: Towards a preliminary model of loss, trauma and recovery

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    The non-offending mother in cases of intrafamilial child sexual abuse has received limited empirical attention in comparative to the considerable body of literature examining victims and perpetrators of child sexual abuse. There is growing evidence that demonstrates that nonoffending mothers’ experience significant loss and trauma following the discovery of their children’s sexual victimisation by a family member, particularly where the perpetrators are their partners. An understanding of the non-offending mother’s experience is crucial to guiding statutory agencies and therapeutic interventions when working with these families. However, there is currently not a model or framework that conceptualises mothers’ post-discovery experience, and the factors that might impede or facilitate their recovery. The aim with the present study was to address the gap in the existing literature, by conducting an exploratory investigation of the lived experience of non-offending mothers in order to generate a preliminary model outlining their recovery journey in the aftermath of discovery, drawing from existing theories of loss and trauma. The present study comprises two stages; in the first stage, qualitative interviews were conducted with a sample of eleven mothers. Data derived from the interviews were analysed using qualitative thematic analysis, from which a preliminary model was generated. The model proposed the non-offending mother’s recovery journey comprises three primary phases; the Acute Phase (Discovery and Destabilisation), the Transition Phase (Loss and Disempowerment), and the Transformative Phase (Taking Control and Accommodation). The preliminary model identified unique aspects of the maternal experience not sufficiently accounted for by many of the existing theoretical conceptualisations. The second stage of the study utilised a Delphi methodology to seek feedback on the proposed model from a panel of 18 key experts in the field of intrafamilial child sexual abuse. The input from the Delphi panel was utilised to further refine and validate the preliminary model. The panel confirmed the preliminary model provided a valid representation of the non-offending mother’s post-discovery experience with minor alterations. The findings of the present study are an important progression towards developing a more comprehensive and unified conceptualisation of the experiences of the non-offending mother in the aftermath of discovery. This in turn has important implications for the intervening professionals from both statutory and therapeutic orientations who work with this population

    Soul Care: Christian Faith and Academic Administration

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    Listening Project

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    In an effort to better serve Southeast and Coastal Georgia, The Coastal Rivers Water Planning & Policy Center tapped the thoughts of several key stakeholders on water issues in our region.The Center was created in 2001 with a continuing mission to "assist policymakers in the formulation of policy designs to best manage sustainable economic growth and natural resource conservation via water planning, research, education and technical assistance." In order to best accomplish this mission, it is necessary for us to engage stakeholders in our region to determine those issues of critical importance.The Listening Project is designed to identify the perspective of water users throughout the Coastal Rivers Region by listening to the actual concerns and ideas for improvement of those who have a stake in the water future of the region. Using this information, the Center can better meet the research needs of stakeholders in the region.The objective of the first round of listening sessions is to identify issues, and not to take a quantitative measure of any given constituency. Thus, the results of the process do not lend themselves to conclusions that any one constituency has a certain viewpoint, but rather gives an idea for the type of issues that arise when representatives of one particular constituency gather to discuss their hopes and fears around the future of water use in Coastal Georgia region.The balance of this paper is organized in the following way: In Section II we discuss the process used in this first round of five listening sessions. In Section III we report the verbatim ideas of the participants in each of the five sessions. Section IV reports the same verbatim ideas put forward by the participants, but the ideas are organized according to dominant themes emerging from the sessions, where various constituencies' ideas on each theme are easily readable in the same place. Finally, in Section V we offer concluding remarks and describe our plans for Phase II of the project. Working Paper Number 2005-00
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