Prophylactic rHA-Infestin-4 application is still active 24 h after tMCAO in rats.

<p>Prophylactic rHA-Infestin-4 application is still active 24 h after tMCAO in rats.</p

Therapeutic treatment with rHA-Infestin-4 improves neurological performance after tMCAO in rats.

<p><b>(A)</b> Zea Longa score 24 h after transient middle cerebral artery occlusion (tMCAO) in rats. Rats were subjected to 90 min of tMCAO followed by reperfusion until 24 h. Rats were treated with 100 mg/kg rHA-Infestin-4 or saline (control) directly after start of reperfusion. Therapeutic rHA-Infestin-4 treatment significantly reduced the Zea Longa score compared to saline treated rats (n = 28-30/group). *p<0.05, Mann-Whitney test compared to saline treated animals. Horizontal solid lines indicate mean values (+SEM). <b>(B)</b> In addition, the Neuroscore was reduced in rHA-Infestin-4 treated rats compared to saline treated rats 24 h after tMCAO (n = 28-30/group). *p<0.05, Mann-Whitney test compared to saline treated animals. Dots above the dashed horizontal line indicate dead animals per group. Mortality was included in statistical analysis. Horizontal solid lines indicate mean values (+SEM) of surviving animals. <b>(C)</b> Functional performance on RotaRod and <b>(D)</b> grip strength in percent compared to the individual baseline of saline treated and rHA-Infestin-4 treated animals 24 h after tMCAO. rHA-Infestin-4 had no effect in these two assays compared to saline treated controls (n = 18-20/ group). p>0.05 between rHA-Infestin-4 treated and saline treated animals, Mann-Whitney test. Dots above the dashed horizontal line indicate dead animals per group. Mortality was included in statistical analysis. Horizontal solid lines indicate mean values (+SEM) of surviving animals.</p