1 research outputs found
Gold Nanoparticles as a Probe for Amyloid‑β Oligomer and Amyloid Formation
The process of amyloid-β (Aβ)
amyloid formation is
pathologically linked to Alzheimer’s disease (AD). The identification
of Aβ amyloids and intermediates that are crucial players in
the pathology of AD is critical for exploring the underlying mechanism
of Aβ aggregation and the diagnosis of the disease. Herein,
we performed a gold nanoparticle (AuNP)-based study to detect the
formation of Aβ amyloid fibrils and oligomers. Our results demonstrate
that the intensity of the surface plasmon resonance (SPR) absorption
band of the AuNPs is sensitive to the quantity of Aβ40 amyloids.
This allows the SPR assay to be used for detection and semiquantification
of Aβ40 amyloids and characterization of the kinetics of Aβ
amyloid formation. Furthermore, our study demonstrates that the SPR
band intensity of the AuNPs is sensitive to the presence of oligomers
of Aβ40, and an Aβ40 mutant which forms more stable oligomers.
The kinetics of the stable oligomer formation of the Aβ40 mutant
can also be monitored following the SPR band intensity change of AuNPs.
Our results indicate that this nanoparticle-based method can be used
for mechanistic studies of early protein self-assembly and fibrillogenesis