Additional file 1 of SPIKE: secure and private investigation of the kidney exchange problem

Additional file 1. Appendix: Supplementary tables

Additional file 1: of Sedation with propofol during ERCP: is the combination with esketamine more effective and safer than with alfentanil? Study protocol for a randomized controlled trial

SPIRIT checklist. (DOC 121 kb

Final risk prediction model of in-hospital mortality after pneumonectomy in the discovery set based on data from 542 consecutive pneumonectomies that were performed between 2003 to 2007.

<p>CI = confidence interval.</p><p>¶ High leukocyte counts were not considered “yes” if related to steroids or immunosuppression with no clinical signs of infection.</p><p>Final risk prediction model of in-hospital mortality after pneumonectomy in the discovery set based on data from 542 consecutive pneumonectomies that were performed between 2003 to 2007.</p

Characteristics of 542 consecutive pneumonectomies from 2003 to 2007 (discovery set).

<p>ASA = American Association of Anesthesiologists. FEV1 = median forced expiratory volume in 1 second. FVC = median forced vital capacity.</p><p>*Pneumonectomies with resections of the atrium, diaphragm, chest wall or superior vena cava were defined as other extensions.</p><p>Characteristics of 542 consecutive pneumonectomies from 2003 to 2007 (discovery set).</p

Characteristics of 232 consecutive pneumonectomies from 2008 to 2010 (validation set).

<p>ASA = American Association of Anesthesiologists.</p><p>Characteristics of 232 consecutive pneumonectomies from 2008 to 2010 (validation set).</p

Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization

We report a microfluidic device, using segmented flow in a two-phase system of immiscible liquids, which delivers aqueous droplets into a modified commercial mass spectrometer. The interface coupling the microfluidics to the mass spectrometer achieves up to 96% sample transfer efficiency to the vacuum chamber. Sample ionization is assisted by multipass infrared laser beam in the interface. The system achieves low femtomole detection limits of several analytes ranging from drugs to proteins. Sample ionization in this segmented-flow sampling was found to be remarkably insensitive to the presence of buffer salts and other matrices

Selected studies of preoperative risk factors for mortality after pneumonectomy.

<p>FEV1/ FVC = forced expiratory volume in 1 second/ forced vital capacity; DLCO = diffusion capacity of lung to carbon monoxide.</p><p>Selected studies of preoperative risk factors for mortality after pneumonectomy.</p

Definitions of the comorbidities according to the International Classification of Diseases (ICD) version 10.

<p>Definitions of the comorbidities according to the International Classification of Diseases (ICD) version 10.</p

Self-digitization chip for single-cell genotyping of cancer-related mutations

<div><p>Cancer is a heterogeneous disease, and patient-level genetic assessments can guide therapy choice and impact prognosis. However, little is known about the impact of genetic variability within a tumor, intratumoral heterogeneity (ITH), on disease progression or outcome. Current approaches using bulk tumor specimens can suggest the presence of ITH, but only single-cell genetic methods have the resolution to describe the underlying clonal structures themselves. Current techniques tend to be labor and resource intensive and challenging to characterize with respect to sources of biological and technical variability. We have developed a platform using a microfluidic self-digitization chip to partition cells in stationary volumes for cell imaging and allele-specific PCR. Genotyping data from only confirmed single-cell volumes is obtained and subject to a variety of relevant quality control assessments such as allele dropout, false positive, and false negative rates. We demonstrate single-cell genotyping of the <i>NPM1</i> type A mutation, an important prognostic indicator in acute myeloid leukemia, on single cells of the cell line OCI-AML3, describing a more complex zygosity distribution than would be predicted via bulk analysis.</p></div

Overview of the single-cell SD genotyping chip workflow.

<p>(<b>a</b>) The chip is composed of PDMS bonded to a microscope slide with a bonded glass coverslip over the array and surrounding oil channel to prevent sample evaporation. (<b>b</b>) Cell-PCR mix suspension is pipetted directly into the inlet reservoir flows into the wells of the array by applying vacuum to the outlet port. Once the sample volume is loaded into the wells, the main channel is flushed with oil to fully digitize each 8nL volume. The arrays are imaged to identify wells containing single (or more) cells. PCR is performed in the digitized volumes by thermalcycling of the entire chip, and fluorescence is quantified at endpoint in three fluorescence channels (FAM for the amplification probe, HEX for the mutant allele-specific probe and Cy5 for the wild-type-specific probe). In wells containing a single-cell and with amplification probe fluorescence above a set threshold, as well as allele-specific probe fluorescence above their respective thresholds, we can generate QC statistics for the array and determine zygosities of single cells.</p