Examples of voltage plots exported form MultiElec.

<p>A) Full length recording for all sixty electrodes. B) A full length recordings for a single electrode. C-E) Sliders enable the user to zoom and focus on a single event/wave front. F) Shows all sixty electrodes zoomed in on a single event. G) Shows single event with calculated activation time marked in green. Smoothed signal superimposed in red.</p

Comparison of beat frequency before and after adrenaline treatment.

<p>A) Full length recording. B) Zoomed recording before addition of adrenaline. C) Zoomed recording after adrenaline. D) Quantitative comparison of beats per min, data represents mean ± SEM. A paired students t-test was performed and the difference found to be significant, *p<0.05, n = 4.</p

Examples of heat maps produced by MultiElec.

<p>A) Heat map with activation times at each electrode. B) Heat map with isolines.</p

Flow chart of MultiElec work flow.

<p>On execution of the program the GUI is opened. The user can then select data to be loaded. Once the data is loaded the program will pre-process the data (filters noise). The user can then select an electrode and focus on a particular signal event. Once a signal event is in view the user can request that activation times are calculated and activation time heat maps are produced. Using these initial heat maps the user can silence problem electrodes and repeat the process until all problem electrodes are silenced. Once appropriate electrodes are silenced conduction, velocities and subsequent velocity vector maps can be produced. At various points during this process figures can be produced and saved for further reference or publication.</p

Assessment of the effect of noise on predicted conduction velocity.

<p>A) FP plots, heat maps and conduction velocity vector maps are shown for a data set with increasing amounts of white noise added. B) Plots of absolute error with indicated noise level when compared to no added noise. Error bars represent SEM, n = 5.</p

Screen shot of MultiElecs’ GUI.

<p>The ‘view electrode’ panel allows the user to select individual electrode to be viewed. The ‘silence electrode’ panel allows the removal of selected electrodes from analysis. The top left plot shows the entire recording for the selected electrode. The top right plot allows the user to zoom in at a chosen time for analysis of the field potential (green dot marks the calculated activation time). The bottom right plot allows the user to view the voltage of the entire set of electrodes change with time.</p

Comparison of conduction velocities before and after adrenaline treatment.

<p>A and B) Heat maps with activation times and conduction velocity vector plots for non-treated and adrenaline-treated cultures, respectively. A and B from two independent experiments.</p

Still frames of a 3D movie recording showing signal progression across electrodes.

<p>9ms recording shows the signal initiation at the bottom left corner of the array and progress outwards.</p