21 research outputs found
Attachment of Pathogenic Prion Protein to Model Oxide Surfaces
Prions are the infectious agents
in the class of fatal neurodegenerative
diseases known as transmissible spongiform encephalopathies, which
affect humans, deer, sheep, and cattle. Prion diseases of deer and
sheep can be transmitted via environmental routes, and soil is has
been implicated in the transmission of these diseases. Interaction
with soil particles is expected to govern the transport, bioavailability
and persistence of prions in soil environments. A mechanistic understanding
of prion interaction with soil components is critical for understanding
the behavior of these proteins in the environment. Here, we report
results of a study to investigate the interactions of prions with
model oxide surfaces (Al<sub>2</sub>O<sub>3</sub>, SiO<sub>2</sub>) using quartz crystal microbalance with dissipation monitoring and
optical waveguide light mode spectroscopy. The efficiency of prion
attachment to Al<sub>2</sub>O<sub>3</sub> and SiO<sub>2</sub> depended
strongly on pH and ionic strength in a manner consistent with electrostatic
forces dominating interaction with these oxides. The presence of the N-terminal portion
of the protein appeared to promote attachment to Al<sub>2</sub>O<sub>3</sub> under globally electrostatically repulsive conditions.
We evaluated the utility of recombinant prion protein as a surrogate
for prions in attachment experiments and found that its behavior differed
markedly from that of the infectious agent. Our findings suggest that
prions would tend to associate with positively charged mineral surfaces
in soils (e.g., iron and aluminum oxides)
Photoluminescence and free carrier interactions in erbium-doped GaAs
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Incorporation of optically active erbium into GaAs using the novel precursor tris(3,5-di-tert-butylpyrazolato)bis(4-tert-butylpyridine)erbium
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder
Long wavelength emission of InGaAsNGaAsSb type II "w" quantum wells
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder
Characteristics of GaAsNGaAsSb type-II quantum wells grown by metalorganic vapor phase epitaxy on GaAs substrates
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder
Atomic Layer Deposited MgO: A Lower Overpotential Coating for Li[Ni<sub>0.5</sub>Mn<sub>0.3</sub>Co<sub>0.2</sub>]O<sub>2</sub> Cathode
An
ultrathin MgO coating was synthesized via atomic layer deposition
(ALD) to improve the surface properties of the LiÂ[Ni<sub>0.5</sub>Mn<sub>0.3</sub>Co<sub>0.2</sub>]ÂO<sub>2</sub> (NMC) cathode. An
in-situ quartz crystal sensor was used to monitor the “self-limiting”
surface reactions during ALD process and estimate the density of the
deposited film. The electrochemical performance of the MgO-coated
NMC cathode was evaluated in a half-cell assembly and compared to
other ALD-based coatings, such as Al<sub>2</sub>O<sub>3</sub> and
ZrO<sub>2</sub>. Cyclic voltammetry studies suggested that ALD MgO
has a higher Li-diffusion coefficient which resulted in lower overpotential
on the NMC cathode surface and improved Li-ion battery rate performance.
MgO-coated NMC also yielded improved capacity retention over uncoated
NMC in a long-range cycling test
Progress towards intersubband quantum-box lasers for highly efficient continuous wave operation in the mid-infrared
Intersubband Quantum-Box (IQB) lasers; that is, devices consisting of 2-D arrays of ministacks (i.e., 2-4 stages) intersubband QB emitters are proposed, as an alternative to 30-stage quantum-cascade (QC) devices, as sources for efficient room-temperature (RT) emission in the mid-infrared (4-6 µm) wavelength range. Preliminary results include: 1) the design of devices for operation with 50% wallplug efficiency at RT; 2) realization of a novel type of QC device: the deep-well (DW) QC laser, that has demonstrated at lambda =4.7µm low temperature sensitivity of the threshold current, a clear indication of suppressed carrier leakage; 3) the formation of 2-D arrays at nanopoles by employing nanopatterning and dry etching; 4) the formation of 40nm-diameter, one-stage IQB structures on 100nm centers by preferential regrowth via metal-organic vapor phase epitaxy (MOVPE).Defence Advanced Research Projects Agency (agreement number FA8650-07-1-7709
Resonantly Enhanced Nonlinear Optical Probes of Oxidized Multiwalled Carbon Nanotubes at Supported Lipid Bilayers
With
production of carbon nanotubes surpassing billions of tons
per annum, concern about their potential interactions with biological
systems is growing. Herein, we utilize second harmonic generation
spectroscopy, sum frequency generation spectroscopy, and quartz crystal
microbalance with dissipation monitoring to probe the interactions
between oxidized multiwalled carbon nanotubes (O-MWCNTs) and supported
lipid bilayers composed of phospholipids with phosphatidylcholine
head groups as the dominant component. We quantify O-MWCNT attachment
to supported lipid bilayers under biogeochemically relevant conditions
and discern that the interactions occur without disrupting the structural
integrity of the lipid bilayers for the systems probed. The extent
of O-MWCNT sorption was far below a monolayer even at 100 mM NaCl
and was independent of the chemical composition of the supported lipid
bilayer