Schematic overview of FAM124B, CHD7 and CHD8 and the constructs used.

<p>In humans, there are two transcript variants of FAM124B. Transcript variant 1 codes for a protein with 455 amino acids (NP_001116251.1), while transcript variant 2 leads to a shorter protein product with 272 amino acids (NP_079061.2). Both variants have the first 244 amino acids in common. All used FAM124B variant 1 and 2 constructs are full length constructs. Bioinformatic analysis of the amino acid sequence of FAM124B failed to identify any known functional domain, while CHD7 and CHD8 consist of two N-terminal chromodomains (Chromo), followed by a SWI2/SNF2-like ATPase/helicase domain (Helicase/ATPase), three conserved regions (CR1-3), a SANT domain and two BRK domains. The black lines mark the regions cloned into the Yeast two hybrid (pGBKT7, pGADT7) and Co-IP (pCMV-HA, pCMV-cmyc) vectors.</p

Fam124B expression in the mouse central nervous system.

<p>(<b>A</b>) Overview, (<b>B</b>) Thalamic nuclei, (<b>C</b>) Hippocampus, (<b>D</b>) Caudate Putamen, (<b>E</b>) Cerebellum. CC = Corpus Callosum, Hipp = Hippocampus, CA1-3 = Cornu Ammonis areas, DG = dentate gyrus, CP = Caudate Putamen, adt = anterior dorsal thalamic nucleus, dg = granual layer of dentate gyrus, lv = lateral ventricle, F = fornix, mdt = mediodorsal thalamic nucleus, IIIv = third ventricle with choroid plexus, vpl = ventral posterior thalamic nucleus, lateral part, ML = molecular layer of cerebellum, PL = purkinje cell layer of cerebellum, GL = granular layer of cerebellum, Scale bar = 100 µm.</p

Co-immunoprecipitation of FAM124B with a part of CHD7 and CHD8.

<p>HeLa cells were co-transfected with either the CHD7-CR1-3-pCMV-HA (amino acids 1593-2178, NP_060250.2) plasmid and FAM124B-1,3-pCMV-cmyc or FAM124B-1,3-pCMV-HA (transcript variant 1, NP_001116251.1) or with CHD8-pCMV-cmyc (amino acids 1789-2302, NP_065971.2) and FAM124B-1,3-pCMV-HA (transcript variant 1, NP_001116251.1). (<b>A</b>) Using the anti-CHD8 (abcam, ab84527) or the anti-CHD7 (abcam, ab31824) antibody for precipitation, we detected with the anti-HA antibody (Roche) an approximately 51 kDa band corresponding to the estimated size of FAM124B transcript variant 1. Lane 1: co-transfected Co-IP, lane 2: untransfected HeLa cells as negative control. (<b>B</b>) Reciprocal immunoprecipitation with anti-cmyc antibody (precipitating FAM124B transcript variant 1), and detection with the anti-CHD7 antibody lead to a specific band ∼70 kDa, the estimated size for the CHD7 part fused to the HA-tag. Lane 1: co-transfected Co-IP, lane 2: untransfected HeLa cells as negative control. (<b>C</b>) Reciprocal experiment with anti-HA antibody (precipitating FAM124B transcript variant 1) and detection with the anti-CHD8 antibody detected a specific band ∼68 kDa, the estimated size for the CHD8 part fused to the cmyc-tag. Lane 1: co-transfected Co-IP, lane 2: untransfected HeLa cells as negative control. (<b>D, E, F</b>) The same experimental procedure was performed for FAM124B transcript variant 2, demonstrating a specific interaction of FAM124B transcript variant 2 with the CHD7 and CHD8 part as well. Lane 1: co-transfected Co-IP, lane 2: untransfected HeLa cells as negative control.</p

Western blot and immunocytochemistry of endogenous and overexpressed FAM124B.

<p>(<b>A</b>) Western blot analysis on protein isolated from untransfected HeLa cells (endogenous FAM124B) and HeLa cells overexpressing the FAM124B-1,3-cmyc-tag fusion protein. Lane 1: Immunoblotting of the nuclear cell fraction of untransfected HeLa cells using the FAM124B antibody. The predicted size of human endogenous FAM124B-1,3 is approximately 51 kDa. However, we observed a band of approximately 57 kDa (labeled by an asterisk). We hypothesize that this band could be endogenous FAM124B and the larger size is possibly due to posttranslational modifications of the endogenous protein. Lane 2: Immunoblotting of FAM124B-1,3-pCMV-cmyc overexpressed HeLa total cell lysate using the rabbit anti-FAM124B antibody revealed a prominent band of 51 kDa, the calculated size of human full length FAM124B variant 1 in fusion with a c-myc-tag and the band observed in untransfected HeLa cells. Lane 3: Immunoblotting of the same HeLa cell lysate as shown in lane 2 using anti-c-Myc shows the 51 kDa band corresponding to the overexpressed FAM124B. Lane 4: Marker. Protein quality was controlled using mouse anti-HSC70 producing a 70 kDa band. (<b>B</b>) Immunofluorescence staining using the rabbit anti-Fam124B antibody on HeLa cells transiently transfected with the plasmid FAM124B-1,3-pCMV-cmyc (FAM124B transcript variant 1 fused to an c-myc-tag). Due to the transient transfection not all cells overexpressed FAM124B. Transfected cells show a bright red signal in the nucleus demonstrating a nuclear distribution. The weaker signal in the cell nuclei of the untransfected surrounding cells could possibly reflect an endogenous FAM124B expression, which we detected by RT-PCR and mass spectrometry. (<b>C</b>) Immunofluorescence staining using the anti-c-Myc antibody on the same HeLa cells transiently transfected with the plasmid FAM124B-1,3-pCMV-cmyc showing a bright green signal in the nucleus of the transfected cells. No signal could be observed in the surrounding untransfected cells. (<b>D</b>) Cell nuclei were stained with DAPI (blue). (<b>E</b>) Overlay of B,C and D. (<b>F</b>) Immunofluorescence staining using the rabbit anti-Fam124B antibody on untransfected HeLa cells revealed a weak nuclear signal as it was observed in the surrounding untransfected cells (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052640#pone-0052640-g005" target="_blank">Figure 5B</a>). (<b>G</b>) Immunofluorescence staining using the anti-c-Myc antibody on untransfected cells reveals no signal, as expected. (<b>H</b>) Staining of cell nuclei with DAPI (<b>I</b>) overlay of F,G and H. B,C,D,E: Scale bar = 40 µm. F,G,H,I: Scale bar = 30 µm.</p

Schematic overview of the SILAC approach.

<p>“Heavy”-labeled cells were co-transfected with the plasmids CHD7-CR1-3-pCMV-HA (containing amino acids 1593-2178, NP_060250.2, in fusion with an HA-tag) and CHD8-pCMV-cmyc (spanning amino acids 1789–2302, NP_065971.2, in fusion with a cmyc-tag). The CHD7 part was purified by anti-HA immunoprecipitation. As a negative control, the same immunoprecipitation was performed in lysates of non-transfected “Light”-labeled HeLa cells. Purified proteins from both cell cultures were pooled in equimolar amounts and in-gel digested, followed by liquid-chromatography (LC)-coupled tandem mass spectrometry.</p

Yeast two hybrid assay.

<p>(<b>A</b>) Direct yeast two hybrid experiment with the constructs FAM124B-1,3-pGADT7 (full length transcript variant 1) and CHD7-CR1-3-pGBKT7 (amino acids 1591-2181, NP_060250.2) demonstrating no direct interaction between FAM124B transcript variant 1 and the CHD7 part, while (<b>B</b>) Direct yeast two hybrid experiment with the constructs FAM124B-1,3-pGADT7 (full length transcript variant 1) and CHD8-pGBKT7 (amino acids 1789–2302, NP_065971.2) shows a direct interaction. The same experiments were performed for FAM124B transcript variant 2. (<b>C</b>) Direct yeast two hybrid experiment with the constructs FAM124B-1,2-pGADT7 (full length transcript variant 2) and CHD7-CR1-3-pGBKT7 (amino acids 1591-2181, NP_060250.2). (<b>D</b>) Direct yeast two hybrid experiment with the constructs FAM124B-1,2-pGADT7 (full length transcript variant 2) and CHD8-pGBKT7 (amino acids 1789–2302, NP_065971.2). FAM124B transcript variant 2 interacts directly with the CHD8 part, while no direct interaction with the CHD7 part could be observed.</p

In situ hybridization of Fam124B on mouse brain cryosections in comparison with immunostaining.

<p>(<b>A</b>) Immunostaining of a cortex section, (<b>B</b>) In situ hybridization (ISH) of a cortex section (<b>C</b>) Sense control of ISH. (<b>D</b>) Immunostaining of a hippocampus section, (<b>E</b>) In situ hybridization of a hippocampus section (<b>F</b>) Sense control of ISH. DG = dentate gyrus. Scale bar = 100 µm.</p