Additional file 2: Figure S2. of Thoughts about SLC16A2, TSIX and XIST gene like sites in the human genome and a potential role in cellular chromosome counting

SLC16A2/TSIX/XIST like sequences through human genome (chromosomes 13–22, X and Y). See figure legend for Additional files 1 and 2: Figures S1-S2. (TIF 3023 kb

Additional file 1: of Application of multicolor banding combined with heterochromatic and locus-specific probes identify evolutionary conserved breakpoints in Hylobates pileatus

The BAC probes applied in the present study. Abbreviations: n.a. = not available; subtel = subtelomeric probe. (DOC 165 kb

Sequence of the B-specific 2200 bp ISSR Amplicon Obtained from gDNA of a Random Animal with 1B Karyotype.

<p>Both, 991 nt at the 5’-end and 995 nt at the 3’-end listed here were completely identical among all ten clones. Approximately 200 intermediate bases without readable sequence results were omitted from the shown sequence. Sequences of eight designed specific primers are underlined: <i>DD5F</i>, <i>DD5R</i>, <i>SR1F</i>, <i>SR1R</i>, <i>SR2R</i>, <i>LD1F</i>, <i>LD1R</i>, and <i>LD2R</i>. Sequences of two fragments amplified by qPCR are colored in orange at 5’ end (321 bp) and green at 3’ end (251 bp).</p

Additional file 1: of Complex intrachromosomal rearrangement in 1q leading to 1q32.2 microdeletion: a potential role of SRGAP2 in the gyrification of cerebral cortex

List of all BACs used in FISH analysis with genomic coordinates (hg38). (DOCX 13 kb

Results of the Calculation of Imported Threshold Cycle (Ct) Data for Two Target Sequences.

<p>Orange 321 bp amplified with <i>DD5F - DD5R</i> primer pair; green 251 bp amplified with <i>LD1F - LD2R</i>.</p

Hybridization of B-marker probe in two metaphases in 1B <i>A</i>. <i>flavicollis</i>.

<p>B chromosome is marked with an arrow.</p

Electrophoretic scan of ISSR profiles amplified with primer (CAG)₄AC.

<p>Lines 1, 2 and 3 obtained by +B gDNA; lines 4, 5 and 6 by 0B. Nt- negative control, M- GeneRuler 100 bp DNA Ladder Plus, Thermo Scientific.</p

RFLP results of mixed digestion of 2200 bp DNA band with <i>Sac</i>I and <i>Hae</i>III.

<p>Lines 2, 4, 6—extracted and purified 2200 bp DNA bands without digestion; lines 3, 5, 7—digested 2200 bp bands; lines 1, 8- GeneRuler 100 bp DNA Ladder Plus, Thermo Scientific.</p

Electrophoretic scan of six PCR products of different lengths.

<p>+B/0B quantitative discrepancy, the first two templates +B and the second two templates 0Bs. L- GeneRuler 100 bp DNA Ladder Plus (Thermo Scientific).</p

Comparison between results of karyotyping and ISSR-PCR profiling with primer (CAG)₄AC on a total sample of 387 individuals of <i>A</i>. <i>flavicollis</i>.

<p>Comparison between results of karyotyping and ISSR-PCR profiling with primer (CAG)₄AC on a total sample of 387 individuals of <i>A</i>. <i>flavicollis</i>.</p