Structure of the work packages (WP) of the DENFREE consortium.

<p>For the DENFREE project work package (WP) 1 (Index case community study of the epidemiology of dengue) is a central WP, which will provide data and biological samples for other WPs. This WP is a multicentric, prospective study in Cambodia and Thailand, which will characterize local DENV transmission patterns, identify subclinical infections for mosquito transmission studies (WP5 entomology), establish empirical mosquito, human density, and geo-spatial data for use in fine-scale and agent-based simulation models (WP3 climate prediction and WP4 epidemiological models), establish a biobank of biological samples from patients, household members, and mosquito vectors for further study in other WPs (WP2 diagnostics, WP6 virology, WP7 immunology, and WP8 human genetics), and test novel diagnostic and prognostic tools developed by WP2. Contributions from each WP will bring complementary help to the consortium to achieve the main aims. WP2 will develop new point-of-care diagnostic tools that can be used in the community to screen subclinical individuals in epidemic regions and to test for DENV in mosquito samples, thereby validating a new mosquito trap tool developed by WP5. WP3 and WP4, by using better surveillance data, will help determine the underlying factors, extent, and course of a DENV epidemic. Altogether, we will provide a new strategy for dengue surveillance for better control of DENV transmission.</p

Supplemental Material - SARS-CoV-2 seroprevalence screening study of a children’s hospital health care workers

Supplemental Material for SARS-CoV-2 seroprevalence screening study of a children’s hospital health care workers by Olivia Pluss, Stephen Berman, Molly Lamb, Vijaya Knight, Yannik Roell, Steven Berkowitz, and Thomas Jaenisch in Journal of Infection Prevention</p

Supplemental Material - SARS-CoV-2 seroprevalence screening study of a children’s hospital health care workers

Supplemental Material for SARS-CoV-2 seroprevalence screening study of a children’s hospital health care workers by Olivia Pluss, Stephen Berman, Molly Lamb, Vijaya Knight, Yannik Roell, Steven Berkowitz, and Thomas Jaenisch in Journal of Infection Prevention</p

Data_Sheet_1_The effect of malaria on childhood anemia in a quasi-experimental study of 7,384 twins from 23 Sub-Saharan African countries.docx

BackgroundYoung children in Sub-Saharan Africa (SSA), particularly those from resource-limited settings, are heavily burdened by anemia and malaria. While malaria infected children frequently become anemic (hemoglobin MethodsWe quantified the malaria-induced effect on hemoglobin levels in children under 5 years of age, leveraging data from 7,384 twins and other multiples, aged 6 to 59 months, from 57 nationally representative Demographic and Health Surveys (DHSs) from 23 SSA countries from 2006 to 2019. The quasi-experimental twin fixed-effect design let us minimize the impact of potential confounders that do not vary between twins.ResultsOur analyses of twins revealed a malaria-induced hemoglobin decrease in infected twins of 9 g/L (95% CI -10; -7, pConclusionEven after rigorous control for confounding through a twin fixed-effects study design, malaria substantially decreased hemoglobin levels among SSA twins, rendering them much more susceptible to severe anemia. This effect reflects the population-level effect of malaria on anemia.</p

Interviews to assess needs and barriers to accessing specimens.

Interviews to assess needs and barriers to accessing specimens.</p

S1 Fig -

A. The Industrial University of Santander, Bucamaranga, Colombia and the Fundaciôn INFOVIDA and Centro de Atención y Diagnóstico de Enfermedades Infecciosas-CDI. B Cayetano Heredia University, Lima, Peru. C. University of Carabobo, Valencia, Venezuela. (DOCX)</p

Governance structure of the ZIKAlliance local biorepositories.

Governance structure of the ZIKAlliance local biorepositories.</p

Common gaps and barriers of high concern for access to specimens.

Common gaps and barriers of high concern for access to specimens.</p

Recommendations regarding features of a VBS.

We present a framework for a federated, virtual biorepository system (VBS) with locally collected and managed specimens, as a ‘global public good’ model based on principles of equitable access and benefit sharing. The VBS is intended to facilitate timely access to biological specimens and associated data for outbreak-prone infectious diseases to accelerate the development and evaluation of diagnostics, assess vaccine efficacy, and to support surveillance and research needs. The VBS is aimed to be aligned with the WHO BioHub and other specimen sharing efforts as a force multiplier to meet the needs of strengthening global tools for countering epidemics. The purpose of our initial research is to lay the basis of the collaboration, management and principles of equitable sharing focused on low- and middle-income country partners. Here we report on surveys and interviews undertaken with biorepository-interested parties to better understand needs and barriers for specimen access and share examples from the ZIKAlliance partnership on the governance and operations of locally organized biorepositories.</div

Virtual biorepository functions and services of greatest benefit listed by order of preferences.

Virtual biorepository functions and services of greatest benefit listed by order of preferences.</p