Rate of neutrophil survival and apoptosis.

<p>Neutrophils isolated from 60 HBD, 44 AASV patients, 8 PV patients, 18 TP, 21 SLE patients, and 20 RA patients were cultured in vitro in AIM-V medium. The percentage of surviving neutrophils (1a) and apoptotic neutrophils (1b) was measured after 20 hours. % Neutrophil survival = % of annexin-V negative and 7-AAD negative cells after 20 hour culture. % Neutrophil apoptosis = % of annexin-V positive and 7-AAD negative cells after 2o hour culture. HBD = healthy blood donors. AASV = ANCA-Associated Systemic Vasculitis. PV = Polycythemia Vera. TP = renal transplant recipients. SLE = Systemic Lupus Erythematosus. RA = Rheumatoid Arthritis.</p

Demographic data for the AASV patients and controls.

<p>AASV = ANCA-associated Systemic Vasculitis. GPA = Granulomatosis with polyangiitis. MPA = Microscopic polyangiitis. N = Number of subjects. F = Female. M = Male. HBD = healthy blood donors. PV = Polycythemia Vera. TP = renal transplant recipients. SLE = Systemic Lupus Erythematosus. RA = Rheumatoid Arthritis. BVAS = Birmingham Vasculitis Activity Score. DAS = Disease activity score. SLEDAI = SLE disease activity index. IQR = Interquartile range.</p

Gene expression of pro-/anti-apoptotic factors in neutrophils.

<p>All results are expressed as median fold change relative to Cyclophilin A.</p>(*)<p>P value<0.05, according to Mann-Whitney test and as compared to HBD.</p><p>HBD = healthy blood donors. AASV = ANCA-associated Systemic Vasculitis. PV = Polycythemia Vera. TP = renal transplant recipients. RA = Rheumatoid Arthritis.</p

Neutrophil survival among patients with high G-CSF levels.

<p>% Neutrophil survival = % of annexin-V negative and 7-AAD negative cells after 20 hour culture. AASV = ANCA-associated Systemic Vasculitis. RA = Rheumatoid Arthritis. GM-CSF = Granulocyte-Colony Stimulating Factor. UD = Undetectable (<2 pg/ml).</p>*/**/***<p>Are signals for the common patients between <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032439#pone-0032439-t002" target="_blank">table 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032439#pone-0032439-t003" target="_blank">3</a>.</p

Gene expression of transcription factors in neutrophils.

<p>All results are expressed as median fold change relative to Cyclophilin A.</p>(*)<p>P value<0.01,</p>(**)<p>p value<0.001, and</p>(***)<p>p value<0.0001, according to Mann-Whitney test and as compared to HBD.</p><p>HBD = healthy blood donors. AASV = ANCA-associated Systemic Vasculitis. PV = Polycythemia Vera. TP = renal transplant recipients. RA = Rheumatoid Arthritis.</p

Neutrophil survival among patients with high GM-CSF levels.

<p>% Neutrophil survival = % of annexin-V negative and 7-AAD negative cells after 20 hour culture. AASV = ANCA-associated Systemic Vasculitis. RA = Rheumatoid Arthritis. GM-CSF = Granulocyte Macrophage-Colony Stimulating Factor. UD = Undetectable (<2 pg/ml).</p>*/**/***<p>Are signals for the common patients between <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032439#pone-0032439-t002" target="_blank">table 2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032439#pone-0032439-t003" target="_blank">3</a>.</p