GENETIC AND PHYLOGENETIC VARIATION IN THE DIFFERENT MOLECULAR FORMS OF MAMMALIAN ERYTHROCYTE CARBONIC ANHYDRASES *

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73183/1/j.1749-6632.1968.tb11878.x.pd

The UPS locus encoding uroporphyrinogen I synthase is located on human chromosome 11

The expression of the UPS locus encoding uroporphyrinogen I synthase has been investigated in human/mouse somatic cell hybrids. Human and mouse uroporphyrinogen I synthase can be readily distinguished by their isoelectric points. In hybrid cells, both human and mouse isozymes are detected. The multiple human uroporphyrinogen I synthase isozymes segregate as a single unit, as expected if they are the products of a single locus. The absence of new heteropolymers in hybrid cells supports the biochemical evidence that the active enzyme is a monomer. The presence of human uroporphyrinogen I synthase in hybrid clones was correlated with the presence of human chromosome 11, or its enzymatic marker, without exception in 44 independent hybrid lines. All other chromosomes could be eliminated as possible locations for this locus, due to their independent segregation. This report represents the first gene assignment for an enzyme in the heme biosynthesis pathway.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/23198/1/0000125.pd