No Longer Home

A father and his wife struggle to adapt to their son just home from the long War on Terrorism. Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers veterans and their family members a customized twelve-week writing class, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences of military experience so both writer and audience may benefit. Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers participants a twelve-week writing class customized for veterans and their family members, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences related to their military experience so the writer and readers may benefit from the stories shared

Navigating Through the Fog of Vertical Merger Law: A Guide to Counselling Hospital-Physician Consolidation under the Clayton Act

Lawyers assessing legality under the antitrust laws of hospital acquisitions of physician practices face a quandary. The case law is sparse, federal enforcement guidance outdated, and academic input conflicting. Applying these muddled standards in the rapidlyevolving health care sector only magnifies the uncertainty. While most transactions will be competitively neutral or beneficial, rapidly evolving market conditions causing integration between hospitals and physicians present opportunities for consolidations that may harm consumer interests. Indeed, given the highly concentrated structure of many hospital markets in the nation, preemptive acquisitions of physician practices may be a tempting strategy for some to undermine competition. This Article offers guidance by analyzing potential theories of competitive harm and addressing factual elements necessary to establish a violation of antitrust merger law

A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins

BACKGROUND: There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to map regions in a protein that are permissive for GFP insertion or to create novel biosensors. While remarkably useful, the current insertional strategies have two major limitations: (1) they only produce one kind, or color, of fluorescent fusion protein and (2) one half of all GFP insertions within the target coding sequence are in the wrong orientation. RESULTS: We have overcome these limitations by incorporating two different fluorescent proteins coding sequences in a single transposon, either in tandem or antiparallel. Our initial tests targeted two mammalian integral membrane proteins: the voltage sensitive motor, Prestin, and an ER ligand gated Ca(2+ )channel (IP(3)R). CONCLUSIONS: These new designs increase the efficiency of random fusion protein generation in one of two ways: (1) by creating two different fusion proteins from each insertion or (2) by being independent of orientation

RISK ANALYSIS OF ADOPTING ZERO RUNOFF SUBIRRIGATION SYSTEMS IN GREENHOUSE OPERATIONS: A MONTE CARLO SIMULATION APPROACH

Zero runoff subirrigation (ZRS) technology can effectively manage fertilizer input while improving greenhouse production efficiency. However, high capital investment costs and inadequate technical information to growers are impediments for adoption. A Monte Carlo simulation was used to compare the profitability and risks of alternative ZRS system investments for greenhouse operations in the northeastern and north central United States. Results showed that the Dutch movable tray system and the flood floor system were most profitable and least risky for small potted plant and bedding crop flat production, respectively. The trough bench system was least favorable because its profitability was low and highly volatile.Risk and Uncertainty,

Ridge, Moldboard, Chisel, and No-Till Effects on Tile Water Quality beneath Two Cropping Systems

Soil conservation tillage systems, including ridge-tillage, often reduce surface water contamination by pesticides because soil erosion and surface runoff are reduced. However, the effects on losses through subsurface drainage tile are somewhat uncertain. Our field study quantified the effects of four tillage practices in continuous corn (Zea mays L.) and corn-soybean [Glycine max (L.) Merr] rotations on herbicide and nitrate N losses in tile drainage water. Fertilizer and pesticide application methods were uniform for ridge, moldboard, chisel, and no-till systems. Pesticide and nitrate N leaching losses were significantly affected by crop rotation. Tillage practice had little influence on nitrate N and pesticide losses to the subsurface drainage water within a corn-soybean rotation. However, ridge-till and no-till resulted in larger losses of atrazine than the moldboard plow and chisel based systems under continuous corn. Tillage system did not affect the timings of peak tile flow occurrences, although peak tile flow volume was affected by tillage, presumably because each system bad its own macropore system related to preservation or annual destruction of biopores by tillage. Corn yields were significantly higher under corn-soybean rotation than with continuous-corn for all tillage practices. These results indicate that continuous corn production is not an environmentally sustainable practice for this area because it resulted in higher nitrate N leaching losses to groundwater, received higher N-applications, and resulted in lower corn yields than the corn-soybean rotation. The results also reinforce the need for studies on chemical placement, rate, and timing for various tillage practices to reduce tile drainage losses of agricultural chemicals

Pepducin-mediated cardioprotection via β-arrestin-biased β2-adrenergic receptor-specific signaling

Reperfusion as a therapeutic intervention for acute myocardial infarction-induced cardiac injury itself induces further cardiomyocyte death. β-arrestin (βarr)-biased β-adrenergic receptor (βAR) activation promotes survival signaling responses in vitro; thus, we hypothesize that this pathway can mitigate cardiomyocyte death at the time of reperfusion to better preserve function. However, a lack of efficacious βarr-biased orthosteric small molecules has prevented investigation into whether this pathway relays protection against ischemic injury in vivo. We recently demonstrated that the pepducin ICL1-9, a small lipidated peptide fragment designed from the first intracellular loop of β2AR, allosterically engaged pro-survival signaling cascades in a βarr-dependent manner in vitro. Thus, in this study we tested whether ICL1-9 relays cardioprotection against ischemia/reperfusion (I/R)-induced injury in vivo. Methods: Wild-type (WT) C57BL/6, β2AR knockout (KO), βarr1KO and βarr2KO mice received intracardiac injections of either ICL1-9 or a scrambled control pepducin (Scr) at the time of ischemia (30 min) followed by reperfusion for either 24 h, to assess infarct size and cardiomyocyte death, or 4 weeks, to monitor the impact of ICL1-9 on long-term cardiac structure and function. Neonatal rat ventricular myocytes (NRVM) were used to assess the impact of ICL1-9 versus Scr pepducin on cardiomyocyte survival and mitochondrial superoxide formation in response to either serum deprivation or hypoxia/reoxygenation (H/R) in vitro and to investigate the associated mechanism(s). Results: Intramyocardial injection of ICL1-9 at the time of I/R reduced infarct size, cardiomyocyte death and improved cardiac function in a β2AR- and βarr-dependent manner, which led to improved contractile function early and less fibrotic remodeling over time. Mechanistically, ICL1-9 attenuated mitochondrial superoxide production and promoted cardiomyocyte survival in a RhoA/ROCK-dependent manner. RhoA activation could be detected in cardiomyocytes and whole heart up to 24 h post-treatment, demonstrating the stability of ICL1-9 effects on βarr-dependent β2AR signaling. Conclusion: Pepducin-based allosteric modulation of βarr-dependent β2AR signaling represents a novel therapeutic approach to reduce reperfusion-induced cardiac injury and relay long-term cardiac remodeling benefits

The 8 o'clock Arc: A Serendipitous Discovery of a Strongly Lensed Lyman Break Galaxy in the SDSS DR4 Imaging Data

We report on the serendipitous discovery of the brightest Lyman Break Galaxy (LBG) currently known, a galaxy at z=2.73 that is being strongly lensed by the z=0.38 Luminous Red Galaxy (LRG) SDSS J002240.91+143110.4. The arc of this gravitational lens system, which we have dubbed the "8 o'clock arc" due to its time of discovery, was initially identified in the imaging data of the Sloan Digital Sky Survey Data Release 4 (SDSS DR4); followup observations on the Astrophysical Research Consortium (ARC) 3.5m telescope at Apache Point Observatory confirmed the lensing nature of this system and led to the identification of the arc's spectrum as that of an LBG. The arc has a spectrum and a redshift remarkably similar to those of the previous record-holder for brightest LBG (MS 1512-cB58, a.k.a "cB58"), but, with an estimated total magnitude of (g,r,i) = (20.0,19.2,19.0) and surface brightness of (mu_g,mu_r,mu_i) = (23.3, 22.5, 22.3) mag/arcsec^2, the 8 o'clock arc is thrice as bright. The 8 o'clock arc, which consists of three lensed images of the LBG, is 162deg (9.6arcsec) long and has a length-to-width ratio of 6:1. A fourth image of the LBG -- a counter-image -- can also be identified in the ARC 3.5m g-band images. A simple lens model for the system assuming a singular isothermal ellipsoid potential yields an Einstein radius of 2.91+/-0.14 arcsec, a total mass for the lensing LRG (within the (10.6+/-0.5)/h kpc enclosed by the lensed images) of 1.04x10^12/h Msun, and a magnification factor for the LBG of 12.3(+15/-3.6). The LBG itself is intrinsically quite luminous (approximately 6L*) and shows indications of massive recent star formation, perhaps as high as 160/h Msun/year.Comment: 4 pages 5 figures, submitted to ApJ Letter

The International Library of Leadership

The International Library of Leadership brings together in one place the most significant writings on leadership, the process by which groups, organizations, and societies seek to satisfy their needs and achieve their objectives. Volume 1 focuses on classic discussions of perennial leadership issues including the moral purpose of leadership, the nature of legitimate authority, and the role of followers. Volume 2 turns to investigations of leadership in the modern era and makes available the seminal social scientific works that inaugurated the modern theories of leadership. Volume 3 builds upon the analyses of power, culture, and gender in the first two volumes to address current ethical, democratic, and international challenges of leadership.https://scholarship.richmond.edu/bookshelf/1002/thumbnail.jp