Production of cellulase by Clostridium papyrosolvens CFR-703

Clostridium papyrosolvens producing filter paperase, carboxymethyl cellulase and cellobiase under anaerobic cultivation conditions at 35°C is described. Higher activities of filter paperase and carboxymethylcellulases wereassayed in 48 h culture filtrate, while maximum cellobiase accumulated in the culture broth at 72 h. Filter paperase,carboxymethylcellulase and cellobiase activities were optimum at 35 °C and pH values of 7.0, 6.5 and 7.5 respectively. Cultivation of the strain in 1000 ml Hungate bottles with 1% cellulose at pH 6.5 and 35°C produced carboxymethyl cellulase, filter paperase and cellobiase activities of 45, 35 and 20 IU/ml respectively