A new lignan glycoside from the aerial parts and cytotoxic investigation of <i>Uvaria rufa</i>

<div><p>Chemical investigation of the aerial parts of <i>Uvaria rufa</i> (Dunal) Blume collected from Vietnam yielded one new lignan glycoside, ufaside (<b>1</b>), along with six known compounds, oxoanolobine (<b>2</b>), ergosta-4,6,8(14),22-tetraen-3-one (<b>3</b>), catechin (<b>4</b>), epicatechin (<b>5</b>), daucosterol (<b>6</b>) and glutin-5-en-3-one (<b>7</b>). Their chemical structures were determined by using NMR, HR-MS spectroscopic analyses and in comparison with the reported data. A cytotoxic analysis of <i>U. rufa</i> herb extracts was performed for the first time using nine human cancer cell lines (MCF-7, MDA-MB-231, LNCaP, MKN7, SW480, KB, LU-1, HepG2 and HL-60) derived from different tumour types. Of these seven constituents, compounds <b>2</b> and <b>3</b> displayed moderate cytotoxicity against the human lung adenocarcinoma cell line (LU-1) with IC₅₀ values of 9.22 ± 1.02 μg/mL and 10.21 ± 1.16 μg/mL, respectively.</p></div

Chemical constituents of <i>Oldenlandia pinifolia</i> and their antiproliferative activities

<p>This study describes the chemical constituents of <i>Oldenlandia pinifolia</i> (Wall. Ex G. Don) Kuntze (synonym <i>Hedyotis pinifolia</i> Wall. Ex G. Don) and discusses their anti-proliferative activities. Thirteen compounds were isolated from the <i>n</i>-hexane, ethyl acetate and <i>n</i>-butanol extracts of whole plants <i>O</i>. <i>pinifolia</i> by chromatography method. Their structures were elucidated using MS and NMR analysis and compared with reported data. They are three anthraquinones, a carotenoid, two triterpenes, four iridoid glycosides and three flavonoid glycosides. Among them, 2-methyl-1,4,6-trihydroxy-anthraquinone is a new one, and three compounds were found for the first time in this genus. MTT assay resulted that the <i>n</i>-butanol extract and four isolated compounds inhibited the proliferation of chronic myelogenous leukaemia cells. The results from Hoechst 33343 staining and caspase 3-inducing exhibited that those four tested compounds induced apoptosis and activated caspase 3 (<i>p</i> < 0.05). One of them, isorhamnetin-3-<i>O</i>-<i>β</i>-rutinoside showed the most activity with IC₅₀ value of 394.68 ± 25.12 μM.</p