GC-MS analysis and In-vitro anti-diabetic activity of ethanolic extract of theAtrocarpus Heterophylus (Unriped Jack Fruit)

Objective: In the present study the ethanolic extracts of Atrocarpus Heterophylus (ETAH) were studied for Aldose reductase, alpha (α)- amylase and alpha (α)-glucosidase inhibition using an in-vitro anti diabetic and evaluate the GC-MS analysis of active compounds of ETAH. Methods: The serial extraction was carried out with a series of solvents: Petroleum ether, ethyl acetate and ethanol with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were subjected to the antidiabetic activity was assessed by employing standard in-vitro techniques. Results and discussion: The result showed ethanolic extract exhibited significant aldose reductase, α-amylase and α-glucosidase inhibitory activities with an all plant extracts respectively and well compared with standard acarbose drug.  GC-MS analysis results revealed that ETAH contain the compounds are Cyclohexanol and Nonadecene. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Conclusion: The investigation confirms that ethanolic extract exhibited highest antidiabetic activity among all extracts, Additional studies on needed for purification, characterization and structural elucidation of bioactive compounds from ethanolic extrac