Experimental study of the flow field inside a whirling annular seal

The flow field inside a whirling annular seal was measured using a 3-D Laser Doppler Anemometer (LDA) system. The seal investigated has a clearance of 1.27 mm, a length of 37.3 mm, and is mounted on a drive shaft with a 50 percent eccentricity ratio. This results in the rotor whirling at the same speed as the shaft rotation (whirl ratio = 1.0). The seal is operated at Reynolds number of 12,000 and a Taylor number of 6,300 (3,600 rpm). The 3-D LDA system is equipped with a rotary encoding system which is used to produce phase averaged measurements of the entire mean velocity vector field and Reynolds stress tensor field from 0.13 mm upstream to 0.13 mm downstream of the seal. The mean velocity field reveals a highly three dimensional flow field with large radial velocities near the inlet of the seal as well as a recirculation zone on the rotor surface. The location of maximum mean axial velocity migrates from the pressure side of the rotor at the inlet to the suction side at turbulence kinetic energy. However, turbulence production and dissipation attain equilibrium fairly quickly with remaining relatively constant over the last half of the seal

Turbulence measurements of high shear flow fields in a turbomachine seal configuration

The mean velocity and Reynolds stress tensor throughout a whirling annular seal are presented. The data was collected with a three dimensional laser Doppler velocimeter using phase averaging. Two axial flow conditions (Re = 12,000 and 24,000) were studied at one shaft speed (Ta = 6,600). The eccentricity and whirl ratios were 50 and 100 percent, respectively. There is a region of high axial momentum in this region is higher in the low Reynolds number case due to an axial recirculation zone that occurs on the suction side of the rotor at the inlet. The recirculation zone does not occur in the high Reynolds number case. At both Reynolds numbers, there is a recirculation zone on the rotor surface in the pressure side of the inlet. This recirculation zone extends from 20 to 200 degrees rotor zenith in the tangential direction, and is one third of a clearance wide radially. The high Reynolds number recirculation zone is 1.5 mean clearances long, while the low Reynolds number zone extends 2 mean clearances downstream. When compared to previous studies, it is apparent that the tangential momentum is no greater for a seal with whirl than for one without if other parameters are constant. Areas of high tangential momentum occur in the clearance where the axial momentum is low. Average exit plane tangential velocities in the high Reynolds number case are 1.5 times greater than those in the other flow case. These results are in general agreement with predictions made by other investigators

An experimental technique for performing 3-D LDA measurements inside whirling annular seals

During the last several years, the Fluid Mechanics Division of the Turbomachinery Laboratory at Texas A&M University has developed a rather unique facility with the experimental capability for measuring the flow field inside journal bearings, labyrinth seals, and annular seals. The facility consists of a specially designed 3-D LDA system which is capable of measuring the instantaneous velocity vector within 0.2 mm of a wall while the laser beams are aligned almost perpendicular to the wall. This capability was required to measure the flow field inside journal bearings, labyrinth seals, and annular seals. A detailed description of this facility along with some representative results obtained for a whirling annular seal are presented

Eccentricity effects upon the flow field inside a whirling annular seal

The flow field inside a whirling annular seal operating at a Reynolds number of 24,000 and a Taylor number of 6600 has been measured using a 3-D laser Doppler anemometer system. Two eccentricity ratios were considered, 0.10 and 0.50. The seal has a diameter of 164 mm, is 37.3 mm long, and has a clearance of 1.27 mm. The rotor was mounted eccentrically on the shaft such that the whirl ratio is 1.0 and the rotor follows a circular orbit. The mean axial velocity is not uniform around the circumference of the seal; near the inlet a region characterized by high velocity of the seal. By the exit, another region of high axial velocity is not uniform around the circumference of the seal; near the inlet a region characterized by high velocity of the seal. By the exit, another region of high axial velocity has developed, this time on the suction side of the seal. The magnitude and azimuthal distance of the migration increased with increasing whirl amplitude (eccentricity). Throughout the seal length, the azimuthal mean velocity varied inversely with the mean axial velocity. Increasing the whirl amplitude did not increase the magnitude of the azimuthal velocity at the seal exit

Identification and genomic location of a reniform nematode (Rotylenchulus reniformis) resistance locus (Renari) introgressed from Gossypium aridum into upland cotton (G. hirsutum)

In this association mapping study, a tri-species hybrid, [Gossypium arboreum × (G. hirsutum × G. aridum)2], was crossed with MD51ne (G. hirsutum) and progeny from the cross were used to identify and map SSR markers associated with reniform nematode (Rotylenchulus reniformis) resistance. Seventy-six progeny (the 50 most resistant and 26 most susceptible) plants were genotyped with 104 markers. Twenty-five markers were associated with a resistance locus that we designated Renari and two markers, BNL3279_132 and BNL2662_090, mapped within 1 cM of Renari. Because the SSR fragments associated with resistance were found in G. aridum and the bridging line G 371, G. aridum is the likely source of this resistance. The resistance is simply inherited, possibly controlled by a single dominant gene. The markers identified in this project are a valuable resource to breeders and geneticists in the quest to produce cotton cultivars with a high level of resistance to reniform nematode

Cytostatic potential of novel agents that inhibit the regulation of intracellular pH

Cells within the acidic extracellular environment of solid tumours maintain their intracellular pH (pHi) through the activity of membrane-based ion exchange mechanisms including the Na+/H+ antiport and the Na+-dependent Cl−/HCO3− exchanger. Inhibition of these regulatory mechanisms has been proposed as an approach to tumour therapy. Previously available inhibitors of these exchangers were toxic (e.g. 4,4-diisothiocyanstilbene-2,2-disulphonic acid), and/or non-specific (e.g. 5-N-ethyl-N-isopropyl amiloride). Using two human (MCF7, MDA-MB231) and one murine (EMT6) breast cancer cell lines, we evaluated the influence of two new agents, cariporide (an inhibitor of the Na+/H+ antiport) and S3705 (an inhibitor of the Na+-dependent Cl−/HCO3− exchanger) on the regulation of intracellular pH (pHi). The cytotoxicity of the two agents was assessed by using clonogenic assays. Our results suggest that cariporide has similar efficacy and potency to 5-N-ethyl-N-isopropyl amiloride for inhibition of Na+/H+ exchange while S3705 is more potent and efficient than 4,4-diisothiocyanstilbene-2,2-disulphonic acid in inhibiting Na+-dependent Cl−/HCO3− exchange. The agents inhibited the growth of tumour cells when they were incubated at low pHe (7.0–6.8), but were non-toxic to cells grown at doses that inhibited the regulation of pHi. Our results indicate that cariporide and S3705 are selective cytostatic agents under in vitro conditions that reflect the slightly acidic microenvironment found in solid tumours