Effect of biofertilizers on horticultural and yield traits in french bean var. Contender under dry temperate conditions of Kinnaur district of Himachal Pradesh

Kinnaur district is known as the dry temperate zone of Himachal Pradesh and is known for off season and quality production of vegetables.In this district of Himachal Pradesh, Natural farming is mostly done with the minimum use of chemical fertilizers. Farmers are unaware of the judicious use of farm yard manure, and biofertilizers due to which yield of the french bean is very low (50-70 q/ha). French bean is one of the most important vegetables intercropped with apple in Kinnaur District. An experiment was conducted during the summer season of 2011 at the Experimental Farm of Vegetable Research Station, Kalpa, Kinnaur, Himachal Pradesh to study the effect of Rhizobium and Phosphorus Solublizing Bacteria (PSB) on the horticultural and yield traits in french bean var. Contender. Six treatments comprising seed treatments (with and without Rhizobium), seed treatment (with and without PSB) along with the combination of 60 % dose of recommended quantity of Calcium Ammonium Nitrate and 75 % dose of recommended quantity of Single Super Phosphate and organic matter were evaluated in a Randomized Complete Block Design (RCBD) with three replications. The results revealed that T5 treatment, i.e. Rhizobium+ PSB+ Organic matter resulted in more number of pods per plant (20), pod length (18 cm) and pod yield/ha (140 q/ha)

AN UPDATE ON NEUROPATHIC PAIN MODELS

An rodent animal model of pain offers a powerful tool in order to understand the mechanism involved in peripheral nerve injury for preclinical study of pain. A battery of neuropathic pain models has been developed to simulate the clinical pain conditions with diverse etiology. This article reviews some of the most widely used or promising new models for chronic pain. Partial spinal ligation, chronic constriction injury, and L5/l6 spinal nerve ligation represent three of the best-characterized rodent models of peripheral neuropathy. For reasons of reproducibility and simplicity, most studies of neuropathic pain are based upon animal models of traumatic nerve injury, usually in the rat sciatic nerve. The present review exhaustively discusses the methodology, behavioral alterations of different animal models of neuropathic pain along with their modiï¬cations. Development of these models has contributed immensely in understanding the chronic pain and underlying peripheral as well as central pathogenic mechanisms.Keywords: Peripheral neuropathy, Neuropathic pain, Chronic constriction injury, Spinal nerve ligation, Partial sciatic nerve ligatio

Immunogenicity of a single dose mRNA vaccine in SARS-CoV-2 exposed subjects: A systematic review

Background: The novel coronavirus is quickly spreading and mutating, putting the public health and lifestyle in shambles. The development and approval of mRNA vaccines came up as a breakthrough. The breadth of immune response after a single-dose vaccination in the already infected population is discovered for understanding the hybrid immunity and side effects associated with second dose. Administering a single-dose vaccine to the seropositive population can spare the doses for the population at higher risk. Methods: PubMed, Web of Science, Google scholar, medRxiv and Cochrane library were explored to extract the original data on the efficacy of single-dose mRNA vaccines in seropositive subjects. The Cochrane risk of bias tool was used to assess the risk of bias in the studies. Results: 6 studies evaluating the immunogenicity of single-dose mRNA vaccine were incorporated along with some observational studies and literature. These studies present promising evidence for administering only single-dose mRNA vaccine in seropositive subjects, providing biphasic immune response of higher breadth and duration. Limitations: Most studies had a small sample size, did not correlate the results with higher age groups, with potential risk factors and the percentage of individuals who contracted breakthrough infections. Conclusions: Single-dose mRNA vaccine can be immunogenic and protective enough for already seropositive population by increasing the number of Spike protein-specific memory B-cells. Vaccination schedules based on existing anti-body titers in such individuals can spare doses for vulnerable groups, especially when there is limited production and supply of vaccines worldwide

Immunogenicity of a single dose mRNA vaccine in SARS-CoV-2 exposed subjects: A systematic review

Background: The novel coronavirus is quickly spreading and mutating, putting the public health and lifestyle in shambles. The development and approval of mRNA vaccines came up as a breakthrough. The breadth of immune response after a single-dose vaccination in the already infected population is discovered for understanding the hybrid immunity and side effects associated with second dose. Administering a single-dose vaccine to the seropositive population can spare the doses for the population at higher risk. Methods: PubMed, Web of Science, Google scholar, medRxiv and Cochrane library were explored to extract the original data on the efficacy of single-dose mRNA vaccines in seropositive subjects. The Cochrane risk of bias tool was used to assess the risk of bias in the studies. Results: 6 studies evaluating the immunogenicity of single-dose mRNA vaccine were incorporated along with some observational studies and literature. These studies present promising evidence for administering only single-dose mRNA vaccine in seropositive subjects, providing biphasic immune response of higher breadth and duration. Limitations: Most studies had a small sample size, did not correlate the results with higher age groups, with potential risk factors and the percentage of individuals who contracted breakthrough infections. Conclusions: Single-dose mRNA vaccine can be immunogenic and protective enough for already seropositive population by increasing the number of Spike protein-specific memory B-cells. Vaccination schedules based on existing anti-body titers in such individuals can spare doses for vulnerable groups, especially when there is limited production and supply of vaccines worldwide

A 54 Mb 11qter duplication and 0.9 Mb 1q44 deletion in a child with laryngomalacia and agenesis of corpus callosum

<p>Abstract</p> <p>Background</p> <p>Partial Trisomy 11q syndrome (or Duplication 11q) has defined clinical features and is documented as a rare syndrome by National Organization of Rare Disorders (NORD). Deletion 1q44 (or Monosomy 1q44) is a well-defined syndrome, but there is controversy about the genes lying in 1q44 region, responsible for agenesis of the corpus callosum. We report a female child with the rare Partial Trisomy 11q syndrome and Deletion 1q44 syndrome. The genomic imbalance in the proband was used for molecular characterization of the critical genes in 1q44 region for agenesis of corpus callosum. Some genes in 11q14q25 may be responsible for laryngomalacia.</p> <p>Results</p> <p>We report a female child with dysmorphic features, microcephaly, growth retardation, seizures, acyanotic heart disease, and hand and foot deformities. She had agenesis of corpus callosum, laryngomalacia, anterior ectopic anus, esophageal reflux and respiratory distress. Chromosome analysis revealed a derivative chromosome 1. Her karyotype was 46,XX,der(1)t(1;11)(q44;q14)pat. The mother had a normal karyotype and the karyotype of the father was 46,XY,t(1;11)(q44;q14). SNP array analysis showed that the proband had a 54 Mb duplication of 11q14q25 and a 0.9 Mb deletion of the submicroscopic subtelomeric 1q44 region. Fluorescence Insitu Hybridisation confirmed the duplication of 11qter and deletion of 1qter.</p> <p>Conclusion</p> <p>Laryngomalacia or obstruction of the upper airway is the outcome of increased dosage of some genes due to Partial Trisomy 11q Syndrome. In association with other phenotypic features, agenesis of corpus callosum appears to be a landmark phenotype for Deletion 1q44 syndrome, the critical genes lying proximal to <it>SMYD3 </it>in 1q44 region.</p

Knowledge, Practices, and Restrictions Related to Menstruation among Young Women from Low Socioeconomic Community in Mumbai, India.

The main objective was to assess knowledge, practices, and restrictions faced by young women regarding their menstrual hygiene. The views of adult women having young daughters were also included and both views were compared. In addition, the factors influencing the menstrual hygiene practices were also studied. The study was carried out during 2008 in Mumbai, India. The mixed methods approach was followed for the data collection. Both qualitative and quantitative methods were used to collect the data. For quantitative survey, totally 192 respondents (96 adult and 96 younger women) were selected. While young women were asked about questions related to their menstruation, adult women were asked questions to find out how much they know about menstrual history of their daughters. The qualitative data helped to supplement the findings from the quantitative survey and to study the factors affecting menstrual practices in young women. The mean age at menarche reported was 13.4 years and 30-40% of young girls did not receive any information about menstruation before menarche. It is thus seen that very few young girls between the age group 15 and 24 years did receive any information before the onset of menstruation. Among those who received some information, it was not adequate enough. The source of information was also not authentic. Both young and adult women agreed on this. Due to the inadequate knowledge, there were certain unhygienic practices followed by the young girls resulting in poor menstrual hygiene. It also leads to many unnecessary restrictions on young girls and they faced many health problems and complaints, which were either ignored or managed inappropriately. The role of health sector was almost negligible from giving information to the management of health problems of these young girls. This paper reemphasizes the important, urgent, and neglected need of providing correct knowledge to the community including adolescent girls

Leukemic transformation in myelodysplastic syndrome: A case report with review of risk factors

Myelodysplastic syndromes are a group of clonal disorders affecting the hemopoietic stem cells and characterized by peripheral&nbsp;cytopenias with normocellular to hypercellular bone marrow and various morphological abnormalities in one or more hemopoietic&nbsp;cell lines. MDS carries a high risk of progression to acute myeloid leukemia especially in subtypes with increased myeloblasts.&nbsp;Here, we present the case of leukemic transformation in MDSin a 41-year-old male who presented with complaints of generalized&nbsp;weakness, loss of appetite for 2 months and fever on and off for 1 week. The patient was diagnosed as MDS-multilineage dysplasia&nbsp;after blood examination and bone marrow biopsy but the patient refused for further treatment

Myelolipoma of the Pelvis: A Case Report and Review of Literature

Myelolipomas are uncommon, benign tumors which typically occur in the adrenal glands and consist of mature adipose tissue and benign hematopoietic components. Myelolipomas can occur outside of the adrenal glands, but the presacral region, retroperitoneum, pelvis, and mediastinum are unusual locations for these tumors. It is important to recognize this entity in these locations since they can attain massive sizes leading to pressure symptoms and need to be differentiated from the malignant tumors like liposarcomas. We present a myelolipoma case in the presacral region. Our case illustrates the clinical approach of these tumors in such unusual locations