Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex

Nucleocytoplasmic transport occurs through the nuclear pore complex (NPC), which in yeast is a highly symmetric ~50 MDa complex consisting of approximately 30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa such as proteins, mRNAs, and ribosomal subunits must be aided across by shuttle proteins (karyopherins, or Kaps). Kap-mediated transport involves FG-nups, a family of NPC proteins. While much has been learned about the mechanism of nucleocytoplasmic transport, many details are still unknown; perhaps among the most important missing details is the binding kinetics of almost all the transport relevant interactions, due to significant technical challenges. The aim of this work is to analyze the protein-protein interactions involved in Kap-mediated transport across the NPC, using biochemical, biophysical, and cell biological approaches. Yeast karyopherins, model cargoes, and full-length FG-nups are enriched from bacteria, and their affinities are studied quantitatively. The presence of competitor proteins and changes in bait protein distribution are seen to effect apparent affinity of these interactions. The relevance of the in vitro Kap/NLS-cargo binding measurements is confirmed with a nucleocytoplasmic import assay that allows quantitative measurements of import to be made within single living cells. Trends observed in vitro for Kap/FG-nup interactions were consistent with ex vivo observations of interactions of transport factors with Xenopus oocyte NPCs and also with in vitro measurements of transport through a synthetic NPC-based filter. This work has suggested a role for factors such as non-specific competition in determining the kinetics and selectivity of transport

Interpolation problems in meteor radar analysis, part 7.6A

Meteor echoes come from random points in the observation volume, and are irregularly spaced in time. This precludes the use of fast fourier transformations (FFT) techniques on the raw data to give the spectrum of waves that are present. One way around this obstacle is to restrict our interest to a particular class of waves, and fit a corresponding model to the raw data. It is assumed that there is no horizontal variation across the observation volume for tides, but in the vertical this is certainly not the case. If, in addition, we are interested in other types of waves which may be present and whose periods are unknown, then examining the raw line-of-sight velocities does not tell us how to modify the model, since the line-of-sight direction is not fixed. This is the motivation for interpolation. Interpolation takes a temporal series of line-of-sight velocities, and transforms it to a temporal series of wind velocities for each orthogonal direction. The velocities along a given direction can then be examined readily for any waves in addition to tides

Artist\u27s Book Ideation Cards

108 cards : color illustrations. Barb Tetenbaum developed the original set of artist\u27s book ideation cards for use in her teaching practice. This version was further developed and designed in collaboration with Julie Chen --Colophon card. Two decks of offset printed playing cards enclosed in an acrylic box. The Artist\u27s Book Ideation Cards deck was designed as an aid to the book artist, to jump start a new project or inform one in progress. There are two decks in the set: Category Cards containing approximately eight cards in each of the seven categories (text, image, structure, paper, layout, technique and color) and 54 Adjective Cards of which three are \u27wild cards.\u27 Choosing a card randomly from each of the seven categories and five cards from the adjectives offers an unexpected recipe for a project --23 Sandy Gallery website. Digitally designed and offset printed. Open edition. Curated title for Fleet Library Special Collections exhibition By Hand: Women & Books Exhibit fall, 2021.https://digitalcommons.risd.edu/specialcollections_artistsbooks/1281/thumbnail.jp

Gymnopaedia, second movement

pamphlet stitch variation with hard cover; cover; title page; interior spread; colophon. Reflecting the music of Erik Satie who composed several Gymnopaedia , an antique ledger forms the visual foundation for playful layers of toe-tapping imagery.https://digitalcommons.risd.edu/specialcollections_artistsbooks/1035/thumbnail.jp

Black Ice and Rain: Psalms 6.6

pamphlet stitch with hard cover and silk covered boards; cover; title page; interior pages; colophon. Michael Donaghy recounts a true story of love and loss, illustrated by Tetenbaum\u27s use of very tactile dyed and collaged handmade paper.https://digitalcommons.risd.edu/specialcollections_artistsbooks/1016/thumbnail.jp

Thermodynamic and nonstoichiometric behavior of the lead-doped and lead-free Bi-2212 systems

EMF measurements of oxygen fugacities as a function of stoichiometry were made on pb-doped and Pb-free superconducting ceramics at 700-815 C using oxygen titration. Equations of oxygen partial pressure vs composition and temperature were derived from the EMF measurements. Thermodynamic assessments of the partial molar quantities {Delta}{bar H}(O{sub 2}) and {Delta}{bar S}(O{sub 2}) for Pb-doped Bi- 2212 and Pb-free Bi-2212 indicate that the solid-state decomposition of these Bi cuprates at low oxygen partial pressure can be represented by the diphasic CuO-Cu{sub 2}O system. Comparison of these results with Pb-doped Bi-2223 in powder and silver sheath form is presented