Thickness-dependent thermal properties of amorphous insulating thin films measured by photoreflectance microscopy

In this work, we report on the measurement of the thermal conductivity of thin insulating films of SiO2 obtained by thermal oxidation, and Al2O3 grown by atomic layer deposition (ALD), both on Si wafers. We used photoreflectance microscopy to determine the thermal properties of the films as a function of thickness in the 2 nm to 1000 nm range. The effective thermal conductivity of the Al2O3 layer is shown to decrease with thickness down to 70% for the thinnest layers. The data were analyzed upon considering that the change in the effective thermal conductivity corresponds to an intrinsic thermal conductivity associated to an additional interfacial thermal resistance. The intrinsic conductivity and interfacial thermal resistance of SiO2 were found to be equal to 0.95 W/m·K and 5.1 × 10− 9 m2K/W respectively; those of Al2O3 were found to be 1.56 W/m·K and 4.3 × 10− 9 m2K/W

Characterization of pairs of toxic and nontoxic misfolded protein oligomers elucidates the structural determinants of oligomer toxicity in protein misfolding diseases

Conspectus: The aberrant misfolding and aggregation of peptides and proteins into amyloid aggregates occurs in over 50 largely incurable protein misfolding diseases. These pathologies include Alzheimer’s and Parkinson’s diseases, which are global medical emergencies owing to their prevalence in increasingly aging populations worldwide. Although the presence of mature amyloid aggregates is a hallmark of such neurodegenerative diseases, misfolded protein oligomers are increasingly recognized as of central importance in the pathogenesis of many of these maladies. These oligomers are small, diffusible species that can form as intermediates in the amyloid fibril formation process or be released by mature fibrils after they are formed. They have been closely associated with the induction of neuronal dysfunction and cell death. It has proven rather challenging to study these oligomeric species because of their short lifetimes, low concentrations, extensive structural heterogeneity, and challenges associated with producing stable, homogeneous, and reproducible populations. Despite these difficulties, investigators have developed protocols to produce kinetically, chemically, or structurally stabilized homogeneous populations of protein misfolded oligomers from several amyloidogenic peptides and proteins at experimentally ameneable concentrations. Furthermore, procedures have been established to produce morphologically similar but structurally distinct oligomers from the same protein sequence that are either toxic or nontoxic to cells. These tools offer unique opportunities to identify and investigate the structural determinants of oligomer toxicity by a close comparative inspection of their structures and the mechanisms of action through which they cause cell dysfunction. This Account reviews multidisciplinary results, including from our own groups, obtained by combining chemistry, physics, biochemistry, cell biology, and animal models for pairs of toxic and nontoxic oligomers. We describe oligomers comprised of the amyloid-β peptide, which underlie Alzheimer’s disease, and α-synuclein, which are associated with Parkinson’s disease and other related neurodegenerative pathologies, collectively known as synucleinopathies. Furthermore, we also discuss oligomers formed by the 91-residue N-terminal domain of [NiFe]-hydrogenase maturation factor from E. coli, which we use as a model non-disease-related protein, and by an amyloid stretch of Sup35 prion protein from yeast. These oligomeric pairs have become highly useful experimental tools for studying the molecular determinants of toxicity characteristic of protein misfolding diseases. Key properties have been identified that differentiate toxic from nontoxic oligomers in their ability to induce cellular dysfunction. These characteristics include solvent-exposed hydrophobic regions, interactions with membranes, insertion into lipid bilayers, and disruption of plasma membrane integrity. By using these properties, it has been possible to rationalize in model systems the responses to pairs of toxic and nontoxic oligomers. Collectively, these studies provide guidance for the development of efficacious therapeutic strategies to target rationally the cytotoxicity of misfolded protein oligomers in neurodegenerative conditions

Entanglement of Dirac fields in non-inertial frames

We analyze the entanglement between two modes of a free Dirac field as seen by two relatively accelerated parties. The entanglement is degraded by the Unruh effect and asymptotically reaches a non-vanishing minimum value in the infinite acceleration limit. This means that the state always remains entangled to a degree and can be used in quantum information tasks, such as teleportation, between parties in relative uniform acceleration. We analyze our results from the point of view afforded by the phenomenon of entanglement sharing and in terms of recent results in the area of multi-qubit complementarity.Comment: 15 pages, with 8 figures (Mar 2006); accepted to Physical Review A, July 2006 - slightly revise

Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments

Response to ‘A well-established fact: Rapid mineralization of organic inputs is an important factor for soil carbon sequestration’ by Angers et al.

Letter to the edito

Feasibility study of a 2 GeV superconducting H−H^{-} linac as injector for the CERN PS

This preliminary feasibility study is based on the availability of the CERN LEP2 superconducting RF system after LEP de-commissioning. The option that is explored is to use this system as part of a high energy H- linac injecting at 2 GeV into the CERN PS, with the aim of reliably providing at its output twice the presently foreseen transverse beam brightness at the ultimate intensity envisaged for LHC. This requires the linac to be pulsed at the PS repetition rate of 0.8 Hz with a mean beam current of 10 mA which is sufficient for filling the PS in 240 ms (i.e. about 100 turns) with the ultimate intensity foreseen for injection for the LHC. The linac is composed of two RFQs with a chopping section, a room temperature DTL, a superconducting section with reduced beta cavities up to 1 GeV, and a section of LEP2 cavities up to 2 GeV. This study deals, in particular, with the problems inherent in H- acceleration up to high energy and in the pulsed operation of SC cavities. Means for compensating microphonic vibrations in the SC cavities are considered, with the aim of reducing the final overall energy spread to the tight requirements for injection into a synchrotron. Other possible applications of such a machine are also briefly reviewed, that make use of its potential for working at a higher duty cycle than required for LHC alone

ULTRA-LOCAL TEMPERATURE MAPPING WITH AN INTRINSIC THERMOCOUPLE

Submitted on behalf of EDA Publishing Association (http://irevues.inist.fr/handle/2042/5920)International audienceWe report on a set-up derived from an Electrostatic Force Microscope (EFM) allowing us to probe temperature with a high spatial resolution. The system uses the well-known Seebeck effect through an intrinsic thermocouple made from an EFM conducting tip put in contact with a conducting sample. The contact radius between tip and sample is currently estimated to be in the 50 to 100 nm range depending on the elastic or the plastic deformation. The contact area can be assimilated to the electrical and thermal contact areas. In those conditions, the issue of heat conduction in air is solved. The thermal measurement is related to the Seebeck junction effect : it will therefore not be sensitive to buried materials or impurities

Report of the study group on a superconducting proton linac as a PS Injector

A proposal was made at the end of 1996 to use the large inventory of RF hardware available after the decommissioning of LEP-2 for the construction of a 2 GeV Superconducting Proton Linac (SPL) to inject directly into the PS [1.1]. The brightness of the beam in the PS at low energy would double, helping the injector complex to satisfy the requirements of the LHC and benefiting the planned proton physics programme. Additional users could also be accommodated thanks to the capability of the SPL to operate at a much larger duty factor than that required for high-energy physics. Consequently, a small study group has been set up to analyse the major technical aspects of the SPL design as well as the processes of injection and capture in the PS. This report summarises the work done so far, and provides some information about the other possible uses of the SPL beam. The feasibility of such a cascade of accelerators is confirmed, although an in-depth design study is still required before the realistic performance and detailed design of that facility can be announced