Trainees' experiences of multidisciplinary public health training schemes in England

From 1999 onwards most English NHS regions launched multidisciplinary public health training schemes. These schemes were open to those from backgrounds other than medicine and followed on from the announcement of a new multidisciplinary Public Health Specialist post-a post equivalent to the traditional medical Consultant in Public Health Medicine. This article documents the issues arising during the first few years of the multidisciplinary public health training schemes. It also includes a number of case studies from trainees who have passed through the training schemes, examining the positive and negative experiences of these trainees. The paper reveals how the schemes initially varied considerably by region, in respect of pay and other terms and conditions. The case studies from ex-trainees reveal a number of positive and negative features of the training schemes. © 2007 The Royal Institute of Public Health

Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System

Components of the renin-angiotensin system (RAS) are expressed by cancer stem cells (CSCs) in many cancer types. We here investigated expression of the RAS by the CSC subpopulations in human head and neck metastatic malignant melanoma (HNmMM) tissue samples and HNmMM-derived primary cell lines. Immunohistochemical staining demonstrated expression of pro-renin receptor (PRR), angiotensin-converting enzyme (ACE), and angiotensin II receptor 2 (AT2R) in all; renin in one; and ACE2 in none of the 20 HNmMM tissue samples. PRR was localized to cells within the tumor nests (TNs), while AT2R was expressed by cells within the TNs and the peritumoral stroma (PTS). ACE was localized to the endothelium of the tumor microvessels within the PTS. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) detected transcripts for PRR, ACE, ACE2, and AT1R, in all the five HNmMM tissue samples and four HNmMM-derived primary cell lines; renin in one tissue sample and one cell line, and AT2R in none of the five HNmMM tissue samples and cell lines. Western blotting showed variable expression of ACE, PRR, and AT2R, but not ACE2, in six HNmMM tissue samples and two HNmMM-derived primary cell lines. Immunofluorescence staining of two HNmMM tissue samples demonstrated expression of PRR and AT2R by the SOX2+ CSCs within the TNs and the OCT4+ CSCs within the PTS, with ACE localized to the endothelium of the tumor microvessels within the PTS