Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments

The proper subcellular localization of RNAs and local translational regulation is crucial in highly compartmentalized cells, such as neurons. RNA localization is mediated by specific cis-regulatory elements usually found in mRNA 3'UTRs. Therefore, processes that generate alternative 3'UTRs-alternative splicing and polyadenylation-have the potential to diversify mRNA localization patterns in neurons. Here, we performed mapping of alternative 3'UTRs in neurites and soma isolated from mESC-derived neurons. Our analysis identified 593 genes with differentially localized 3'UTR isoforms. In particular, we have shown that two isoforms of Cdc42 gene with distinct functions in neuronal polarity are differentially localized between neurites and soma of mESC-derived and mouse primary cortical neurons, at both mRNA and protein level. Using reporter assays and 3'UTR swapping experiments, we have identified the role of alternative 3'UTRs and mRNA transport in differential localization of alternative CDC42 protein isoforms. Moreover, we used SILAC to identify isoform-specific Cdc42 3'UTR-bound proteome with potential role in Cdc42 localization and translation. Our analysis points to usage of alternative 3'UTR isoforms as a novel mechanism to provide for differential localization of functionally diverse alternative protein isoforms

The diversity of data needed to drive design

The developers of the Refugee Housing Unit know every aspect and component of their design but can never know what it is like to wake up in one of them every day. Likewise, the end user does not have the tools or resources to make comprehensive changes to its design. The point is about how to work together on it

An intelligent multi-agent system using fuzzy analytic hierarchy process and axiomatic design as a decision support method for refugee settlement siting

Crises and disasters of recent years are complex and occasionally interrelated phenomena, which require complex decision making for effective humanitarian relief operations provision. Moreover, coordination is needed between different humanitarian actors as decision makers. Massive refugee and migrant arrivals in Greece since 2014, mainly a result of a refugee crisis, require complex humanitarian supply chain management and logistics operations. In this paper, refugee settlement site planning decision making process is addressed with an intelligent multi-agent system (MAS) modeling approach. The MAS uses two multi-criteria decision making (MCDM) methods: fuzzy analytic hierarchy process (FAHP) to determine the weights of criteria and fuzzy axiomatic design approach extended with risk factors (RFAD) to rank alternative sites for refugee settlement. The proposed method will be applied to evaluate currently operating temporary accommodation sites in Greece. © Springer International Publishing AG, part of Springer Nature 2018

Commercial building at 4854 S. State Street built in the 1920s

Vine St. Antiques owned by Deniro Enterprises. The Murray State Bank was located here in the 1920s and later in the 1950s by Continental Bank. Later occupants included Olaf Sundwall medical offices and Delbert Wolfley's Murray Office Supply