Influence of nanoparticles of platinum on chicken embryo development and brain morphology

Platinum nanoparticles (NP-Pt) are noble metal nanoparticles with unique physiochemical properties that have recently elicited much interest in medical research. However, we still know little about their toxicity and influence on general health. We investigated effects of NP-Pt on the growth and development of the chicken embryo model with emphasis on brain tissue micro- and ultrastructure. The embryos were administered solutions of NP-Pt injected in ovo at concentrations from 1 to 20 μg/ml. The results demonstrate that NP-Pt did not affect the growth and development of the embryos; however, they induced apoptosis and decreased the number of proliferating cells in the brain tissue. These preliminary results indicate that properties of NP-Pt might be utilized in brain cancer therapy, but potential toxic side effects must be elucidated in extensive follow-up research

Effects of Starter Diets on Pancreatic Enzyme Activity in Juvenile Sterlet (Acipenser ruthenus)

Nine-day-old sterlet (Acipenser ruthenus) larvae were reared for 21 days on one of three commercial diets with different protein and fat contents: Bio Kyowa, Aglo Norse, or Perla Larva Proactive. At the end of the experiment, the sterlet juveniles were sampled for histological analysis of the pancreas and evaluation of lipase, trypsin, and amylase activity. Fish fed the Bio Kyowa and Aglo Norse diets were the largest. Survival was highest in the Aglo Norse group. There were no differences between groups in histologi- cal analysis of the pancreas, and no histological anomalies. The highest lipase activity was observed in fish fed the diet with the highest lipid content (21%) - the Aglo Norse diet. Trypsin activity was higher in fish fed Bio Kyowa with a protein content of 55% than in fish fed Aglo Norse with a pro- tein content of 59%

Akwakultura jako narzędzie poprawy statusu karpiowatych ryb reofilnych w Polsce – stan i perspektywy badań

Ekosystemy śródlądowych wód płynących na skutek przekształceń o charakterze antropogenicznym są obecnie jednymi z najbardziej zmienionych i zagrożonych środowisk naturalnych. Ichtiofauna, jako istotny element biosystemu, również podlega tym zmianom. W efekcie, od wielu lat obserwuje się zmniejszenie liczebności ugrupowań ryb zasiedlających rzeki. Karpiowate ryby reofilne są ważnym składnikiem ichtiocenoz rzecznych. Ze względu na znaczenie środowiskowe, ale również gospodarcze, od wielu lat są obiektem zainteresowań zespołów badawczych w Polsce. W latach osiemdziesiątych XX wieku rozpoczęto programy badawcze ukierunkowane na opracowanie metod rozrodu oraz wychowu materiału zarybieniowego tej grupy ryb. Badania te zakończyły się sukcesem naukowym, a także aplikacyjnym. W wyniku ponad dwudziestoletnich zabiegów nastąpiła poprawa statusu niektórych opisywanych gatunków w zakresie stopnia ich zagrożenia. Obecnie istnieje konieczność kontynuowania intensywnych działań zmierzających do ochrony i odbudowy populacji tych gatunków ryb reofilnych, których status nie uległ poprawie. Jest to możliwe między innymi dzięki opracowaniu innowacyjnych biotechnik w akwakulturze. Mimo wielu zagrożeń, akwakultura karpiowatych ryb reofilnych oparta na wynikach badań naukowych może stać się w większym zakresie niż obecnie ważnym elementem nowoczesnej akwakultury w Polsce, wspierającej działania na rzecz ochrony i poprawy zasobów przyrodniczych i zachowania różnorodności biologicznej wód płynących

The Influence of Diet Containing Wheat Gluten Supplemented with Dipeptides or Amino Acids on the Morphology of White Muscle of Yellow Perch (Perca flavescens)

Nutrition affects the metabolism of muscle cells and myogenic progenitor cells which play a crucial role in the growth and development of the muscle tissue. Because of the fact that the development process of yellow perch muscle tissue is not well known, the study aimed to analyze the influence of diets containing wheat gluten and supplemented with Lys and Gly in dipeptides or free form. Fish were allocated into 12 tanks and divided into four groups. Two of the experimental diets were supplemented Lys-Gly in the dipeptide form (DP group) or free amino acids (FAA group). The third was not supplemented with lysine (LF group). The fourth group of fish was fed commercial starter Bio-Oregon (C group). Histological or histomorphometric analyses were conducted: white muscle area, the total number of muscle fibers, the total number of white muscle nuclei, muscle fiber area, number of proliferating myonuclei. Fish fed LF diet showed the lowest number of nuclei and satellite cells proliferation. Results in DP and FAA groups were similar to that observed in fish fed C diet. Summarizing, wheat gluten-based diets supplemented with Lys-Gly dipeptide or free Lys and Gly amino acids exert beneficial effects on the morphology of yellow perch white muscle

Effect of Muscle Extract and Graphene Oxide on Muscle Structure of Chicken Embryos

SIMPLE SUMMARY: Genetic selection of broilers increased muscle growth; however, very fast growth can lead to pathological conditions caused by the deficiency of nutrients. The number of muscle cells is mainly formed during the embryonic period, and consequently, in ovo supplementation of proteins to embryos may impact future muscle structure. We hypothesized that proteins from chicken embryo muscle extract (CEME) caused by the unique, natural composition and biocompatibility can supply additional proteins. However, supplemented proteins are actively metabolized, which may reduce their utilization for improved muscle synthesis. Nevertheless, CEME can be transported and protected by graphene oxide (GO). The objective of the present work was to investigate the effects of in ovo-injected CEME and the complex of GO-CEME on embryonic cell cultures and the growth of chicken embryo hind limb muscle. Toxicity and cell proliferation were measured in vitro with cell cultures and mortality, morphology, histology, and blood biochemistry in vivo with embryos. CEME increased the number of cells and nuclei in muscle, but the complex GO-CEME did not further improve the muscle structure. The results indicate a vital role of CEME as in ovo enhancer of muscle development in broilers. ABSTRACT: The effects of CEME and it complex with GO injected in ovo on the growth and development of chicken embryo hindlimb muscle were investigated. First, the preliminary in vitro study on primary muscle precursor cell culture obtained from a nine-day-old chicken embryo was performed to assess toxicity (MTT assay) of CEME, GO (100 ppm) and it complex with different concentrations (1, 2, 5, and 10 wt.%). The effect on cell proliferation was investigated by BrdU assay. CEME at concentrations 1–5% increased cell proliferation, but not the complex with GO. In vitro cytotoxicity was highest in 10% and GO groups. Next, the main experiment with chicken embryos was performed with CEME, GO and it complex injected in ovo on day one of embryogenesis. On day 20 of embryogenesis survival, morphological development, histological structure of the muscle, and biochemical parameters of blood serum of the embryos were measured. No negative effect on mortality, body weight, or biochemistry of blood after use of CEME or GO-CEME complexes was observed. Interestingly, the slight toxicity of GO, observed in in vitro studies, was not observed in vivo. The use of CEME at the levels of 2% and 5% improved the structure of the lower limb muscle by increasing the number of cells, and the administration of 2% CEME increased the number of nuclei visible in the stained cross-section of the muscle. The complex GO-CEME did not further improve the muscle structure. The results indicate that CEME can be applied as an in ovo enhancer of muscle development in broilers